| Rabbit Anti-ITGB3 antibody |
| 反应物种（预测） |
Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep |
| 产品应用（已验证） |
WB,FCM |
| 产品应用（可尝试） |
ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,Flow Cyt=1ug/Test, |
| 研究领域 |
细胞生物,免疫学,信号转导,干细胞,细胞粘附分子, |
| 标签 |
Array |
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Sample:
Lane 1: Thyroid gland (Mouse) Lysate at 40 ug
Lane 2: Jurkat (Human) Cell Lysate at 30 ug
Lane 3: Du145 (Human) Cell Lysate at 30 ug
Lane 4: Huvec (Human) Cell Lysate at 30 ug
Primary: Anti-Integrin beta 3 (bs-24402R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 100 kD
Observed band size: 110 kD
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Blank control:HUVEC.
Primary Antibody (green line): Rabbit Anti-Integrin beta 3 antibody (bs-24402R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID：RRID
产品名称：Rabbit Anti-ITGB3 antibody
别名： Integrin beta-3; Integrin beta chain, β3 precursor; Integrin Beta 3; CD 61; CD61; CD61 antigen; GP3A; GPIIIa; HPA 1; HPA 4; Integrin beta 3 (platelet glycoprotein IIIa antigen CD61); Integrin beta chain beta 3; ITG B3; ITGB 3; NAIT; Platelet fibrinogen re
中文名称：整合素β3/CD61抗体
英文名称：Rabbit Anti-ITGB3 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human Integrin beta 3
抗原表位：251-350/788
SWISS：P05106
Gene ID ：3690
Human Gene ID：3690
The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. [provided by RefSeq, Jul 2008]
Function：Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin,
Subunit：Heterodimer of an alpha and a beta subunit. Beta-3 associates with either alpha-IIb or alpha-V. Isoform Beta-3C interacts with FLNB. Interacts with COMP. Interacts with HIV-1 Tat. Interacts with PDIA6 following platelet stimulation. Interacts with SYK; up
Subcellular Location：Membrane; Single-pass type I membrane protein.
Tissue Specificity：Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.
Post-translational modifications：Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation o
DISEASE：Defects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous b
Similarity：Belongs to the integrin beta chain family. Contains 1 VWFA domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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