| Rabbit Anti-SIRT3 antibody |
| 反应物种（预测） |
Rat |
| 产品应用（已验证） |
WB,IHC,ICC |
| 推荐稀释比例 |
WB=1:500-1000,IHC-P=1:20-50,IHC-F=1:20-50,ICC=1:100, |
| 研究领域 |
肿瘤,心血管,细胞生物,细胞周期蛋白,线抗体, |
| 标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SIRT3) Polyclonal Antibody, Unconjugated (bs-55199R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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NIH/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (KO Validated)SIRT3 polyclonal Antibody, Unconjugated (bs-55199R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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Paraformaldehyde-fixed, paraffin embedded (human thyroid cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SIRT3) Polyclonal Antibody, Unconjugated (bs-55199R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SIRT3) Polyclonal Antibody, Unconjugated (bs-55199R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Sample:
Lane 1: 293T (Human) Cell Lysate at 25 ug
Lane 2: SIRT3 knockout (KO) 293T (Human) Cell Lysate at 25 ug
Primary: Anti-SIRT3 (bs-55199R) at 1/1000 dilution
Secondary: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution
Predicted band size: 28 kD
Observed band size: 30 kD
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Sample:
Lane 1: Panc-1 (Human) Cell Lysate at 30 ug
Lane 2: 293T (Human) Cell Lysate at 30 ug
Lane 3: K562 (Human) Cell Lysate at 30 ug
Lane 4: HepG2 (Human) Cell Lysate at 30 ug
Primary:
Anti-SIRT3 (bs-55199R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
RRID：RRID
产品名称：Rabbit Anti-SIRT3 antibody
别名： hSIRT3; hSIRT 3; hSIRT3; Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase; NAD dependent deacetylase sirtuin 3 mitochondrial; NAD-dependent deacetylase sirtuin-3, mitochondrial; Silent mating type information regulation 2 S.cerevisiae
中文名称：线粒体乙酰化酶3抗体
英文名称：Rabbit Anti-SIRT3 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant
免 疫 原：KLH conjugated synthetic peptide derived from human SIRT3
抗原表位：300-399/399
SWISS：Q9NTG7
Gene ID ：23410
Human Gene ID：23410
SIRT3 encodes a member of the sirtuin family of class III histone deacetylases, homologs to the yeast Sir2 protein. The encoded protein is found exclusively in mitochondria, where it can eliminate reactive oxygen species, inhibit apoptosis, and prevent the formation of cancer cells. SIRT3 has far-reaching effects on nuclear gene expression, cancer, cardiovascular disease, neuroprotection, aging, and metabolic control. [provided by RefSeq, May 2019]
Function：NAD-dependent protein deacetylase. Activates mitochondrial target proteins, including ACSS1, IDH2 and GDH by deacetylating key lysine residues. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP l
Subunit：Interacts with NDUFA9, ACSS1, IDH2 and GDH.
Subcellular Location：Mitochondrion matrix.
Tissue Specificity：Widely expressed.
Post-translational modifications：Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.
Similarity：Belongs to the sirtuin family. Contains 1 deacetylase sirtuin-type domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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