Rabbit Anti-NDRG1 antibody |
反应物种(预测) |
Mouse,Rat |
产品应用(已验证) |
WB |
推荐稀释比例 |
WB=1:500-2000, |
研究领域 |
细胞生物,神经生物学,转运蛋白, |
标签 |
Array |
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Sample:
Lane 1: HT1080 (Human) Cell Lysate at 30 ug
Lane 2: U87MG (Human) Cell Lysate at 30 ug
Lane 3: 293T (Human) Cell Lysate at 30 ug
Primary: Anti-NDRG1 (bs-55141R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 45 kD
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Sample:
Lane 1: 293T (Human) Cell Lysate at 25 ug
Lane 2: NDRG1 knockout (KO) 293T (Human) Cell Lysate at 25 ug
Primary: Anti-NDRG1 (bs-55141R) at 1/1000 dilution
Secondary: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
RRID:RRID
产品名称:Rabbit Anti-NDRG1 antibody
别名: Cap43; N-myc downstream regulated gene 1; TDD5; 42 kDa; cap43; cmt4d; Differentiation related gene1 protein; Drg 1; drg1; gc4; hmsnl; Human mRNA for RTP complete cds; N myc downstream regulated gene 1 protein; Ndr 1; NDRG 1; Nickel specific induction prot
中文名称:分化相关基因NDRG1抗体
英文名称:Rabbit Anti-NDRG1 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞核,细胞浆,细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant
免 疫 原:Recombinant human NDRG1
抗原表位:175-394/394
SWISS:Q92597
Gene ID :10397
Human Gene ID:10397
This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
Function:Stress-responsive protein involved in hormone responses, cell growth, and differentiation. Acts as a tumor suppressor in many cell types. Necessary but not sufficient for p53/TP53-mediated caspase activation and apoptosis. Has a role in cell trafficking,
Subunit:Interacts with RAB4A (membrane-bound form); the interaction involves NDRG1 in vesicular recycling of CDH1.
Subcellular Location:Cytoplasm, cytosol. Cytoplasm, cytoskeleton, centrosome. Nucleus. Cell membrane. Note=Mainly cytoplasmic but differentially localized to other regions. Associates with the plasma membrane in intestinal epithelia and lactating mammary gland. Translocated t
Tissue Specificity:Ubiquitous; expressed most prominently in placental membranes and prostate, kidney, small intestine, and ovary tissues. Also expressed in heart, brain, skeletal muscle, lung, liver and pancreas. Low levels in peripheral blood leukocytes and in tissues of
Post-translational modifications:Under stress conditions, phosphorylated in the C-terminal on many serine and threonine residues. Phosphorylated in vitro by PKA. Phosphorylation enhanced by increased intracellular cAMP levels. Homocysteine induces dephosphorylation. Phosphorylation by SG
DISEASE:Defects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D) [MIM:601455]; also known as hereditary motor and sensory neuropathy Lom type (HMSNL). CMT4D is a recessive form of Charcot-Marie-Tooth disease, the most common inherited disorde
Similarity:Belongs to the NDRG family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.