| Rabbit Anti-SOCS3 antibody |
| 反应物种（预测） |
Dog,Pig,Cow,Horse,Rabbit,Sheep |
| 产品应用（已验证） |
WB,FCM |
| 推荐稀释比例 |
WB=1:500-2000,Flow Cyt=1ug/Tes, |
| 研究领域 |
细胞生物,免疫学,神经生物学,信号转导 |
| 标签 |
Array |
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Blank control:U937.
Primary Antibody (green line): Rabbit Anti-SOCS3 antibody (bs-24250R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Sample:
Lane 1: Heart (Mouse) Lysate at 40 ug
Lane 2: Kidney (Mouse) Lysate at 40 ug
Lane 3: Cerebrum (Rat) Lysate at 40 ug
Lane 4: Lymph node (Rat) Lysate at 40 ug
Primary: Anti-SOCS3 (bs-24250R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23-27 kD
Observed band size: 27/23 kD
RRID：RRID
产品名称：Rabbit Anti-SOCS3 antibody
别名： suppressor of cytokine signaling 3; ATOD4; CIS 3; CIS3; Cish3; Cytokine induced SH2 protein 3; E2a Pbx1 target gene in fibroblasts 10; EF 10; MGC71791; SOCS 3; SOCS-3; SSI 3; SSI3; STAT induced STAT inhibitor 3; Suppressor of cytokine signaling 3; SOCS3_H
中文名称：细胞因子信号传导抑制蛋白3抗体
英文名称：Rabbit Anti-SOCS3 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of ant
免 疫 原：KLH conjugated synthetic peptide derived from human SOCS3
抗原表位：161-225/225
SWISS：O14543
Gene ID ：9021
Human Gene ID：9021
Cytokines signal transduction pathway suppressor of cytokine signaling 3 positive cells in the brain are widely distributed, some of the cytoplasm of neurons, nerve fibers, glial cell line. suppressor of cytokine signaling 3 studies in recent years to prove: vascular endothelial cells also showed immune SOCS 3-positive.
Function：SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by bi
Subunit：Interacts with multiple activated proteins of the tyrosine kinase signaling pathway including IGF1 receptor, insulin receptor and JAK2. Binding to JAK2 is mediated through the KIR and SH2 domains to a phosphorylated tyrosine residue within the JAK2 JH1 do
Tissue Specificity：Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.
Post-translational modifications：Phosphorylated on tyrosine residues after stimulation by the cytokines, IL-2, EPO or IGF1.
DISEASE：Genetic variation in the promoter region of SOCS3 may be associated with susceptibility to atopic dermatitis 4 (ATOD4) [MIM:605805]. Atopic dermatitis [MIM:603165], also known as eczema commonly begins in infancy or early childhook and is characterized by
Similarity：Contains 1 SH2 domain.
Contains 1 SOCS box domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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