Rabbit Anti-Tubulin beta-III antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

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重组兔单抗

Rabbit Anti-Tubulin beta-III antibody

单克隆 | CloneNo.: 3B7 | SKU：bsm-52323R

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货号:bs-3351R

￥1280

50μg

RMB1280

100μg

RMB1980

200μg

RMB2980

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产品信息
免疫原信息
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订购号:bsm-52323R

￥1580.00-2680.00

50ul

100ul

货期：现货

售后保障

Rabbit Anti-Tubulin beta-III antibody
反应物种（预测）	Rat
产品应用（已验证）	WB,IHC,ICC,FCM
产品应用（可尝试）	IF
推荐稀释比例	WB=1:1000-2000,IHC-P=1:50-200,IHC-F=1:50-200,Flow Cyt=1:50,IF=1:50-200,ICC=1:50,
研究领域	细胞生物,神经生物学,细胞骨架,
标签	Array

SHG-44 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Tubulin beta-III (3B7)) Monoclonal Antibody, Unconjugated (bsm-52323R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Tubulin beta-III (3B7)) Monoclonal Antibody, Unconjugated (bsm-52323R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tubulin beta-III) Monoclonal Antibody, Unconjugated (bsm-52323R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control:N2A.
Primary Antibody (green line): Rabbit Anti-TUBB3 antibody (bsm-52323R)
Dilution: 1:50;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1:1000.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Sample:
Lane 1: PC-12 cell lysate
Lane 2: Hela cell lysate
Lane 3: SH-SY5Y cell lysate
Primary: Anti-Tubulin beta-III (bsm-52323R) at 1:500 dilution
Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD

产品信息

RRID：RRID
产品名称：Rabbit Anti-Tubulin beta-III antibody
别名： Neuron specific beta III Tubulin; beta 4; MC1R; TBB3_HUMAN; TUBB 3; TUBB 4; TUBB3; TUBB4; Tubulin beta 3 chain; Tubulin beta 4; Tubulin beta III; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III; Beta tubulin III; Neuron specific beta III Tubu
中文名称：微管蛋白β3重组兔单克隆抗体
英文名称：Rabbit Anti-Tubulin beta-III antibody
抗体来源： Rabbit
克隆类型：单克隆
细胞定位：细胞浆
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated synthetic peptide derived from human Tubulin beta-III
SWISS：Q13509
Gene ID ：10381
Human Gene ID：10381

产品介绍

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
Function：Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
Subcellular Location：Cytoplasm, cytoskeleton.
Tissue Specificity：Expression is primarily restricted to central and peripheral nervous system.
Post-translational modifications：Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of fu
DISEASE：Defects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or troc
Similarity：Belongs to the tubulin family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.