| Rabbit Anti-Mast Cell Tryptase antibody |
| 反应物种（预测） |
Mouse,Rat |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
IP |
| 推荐稀释比例 |
WB=1:500-2000,IP=1:10-50,IHC-P=1:50-200,IHC-F=1:50-200, |
| 研究领域 |
细胞生物,免疫学,激酶和磷酸酶 |
| 标签 |
Array |
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Sample:
Lane 1: human skin tissue lysates
Primary: Anti-Mast Cell Tryptase (bsm-52533R) at 1:500 dilution
Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution
Predicted band size: 23 kD
Observed band size: 30 kD
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Paraformaldehyde-fixed, paraffin embedded (human lung tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Mast Cell Tryptase) Monoclonal Antibody, Unconjugated (bsm-52533R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (human prostate carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Mast Cell Tryptase) Monoclonal Antibody, Unconjugated (bsm-52533R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (human small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Mast Cell Tryptase) Monoclonal Antibody, Unconjugated (bsm-52533R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Mast Cell Tryptase) Monoclonal Antibody, Unconjugated (bsm-52533R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID：RRID
产品名称：Rabbit Anti-Mast Cell Tryptase antibody
别名： Tryptase delta; Delta-tryptase; HmMCP-3-like tryptase III; Mast cell mMCP-7-like; Tryptase-3; TPSD1; MCP7L1; MMCP-7L; MCP7-LIKE; EC 3.4.21.59; MGC95428; TRYD; Tryptase3; alpha II; EC 3.4.21.59; Lung tryptase; Mast cell alpha II tryptase; Mast cell beta I
中文名称：肥大细胞类胰蛋白酶重组兔单克隆抗体
英文名称：Rabbit Anti-Mast Cell Tryptase antibody
抗体来源： Rabbit
克隆类型：单克隆
细胞定位：分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：Recombinant human Mast Cell Tryptase
SWISS：Q15661
Gene ID ：7177
Human Gene ID：7177
Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. Although this gene may be an exception, most of the tryptase genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. This gene was once considered to be a pseudogene, although it is now believed to be a functional gene that encodes a protein. [provided by RefSeq, Jul 2008]
Function：Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type.
Subunit：Homotetramer.
Subcellular Location：Secreted. Note=Released from the secretory granules upon mast cell activation.
Tissue Specificity：Expressed in colon, lung, heart and synovial tissue. May be specific to mast cells.
Similarity：Belongs to the peptidase S1 family. Tryptase subfamily.
Contains 1 peptidase S1 domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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