| Rabbit Anti-RNF105 antibody |
| 反应物种（预测） |
Human |
| 产品应用（已验证） |
IHC |
| 推荐稀释比例 |
IHC-P=1:100-500, |
| 研究领域 |
细胞生物,神经生物学,信号转导,G蛋白信号, |
| 标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF105) Polyclonal Antibody, Unconjugated (bs-22472R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
-
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF105) Polyclonal Antibody, Unconjugated (bs-22472R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID：RRID
产品名称：Rabbit Anti-RNF105 antibody
别名： DCRR1; E3 ubiquitin protein ligase TTC3; E3 ubiquitin-protein ligase TTC3; Protein DCRR1; RING finger protein 105;Tetratricopeptide repeat protein 3 (TPR repeat protein D); Tetratricopeptide repeat protein 3; TPR repeat protein 3; TPR repeat protein D; TP
中文名称：环指蛋白105抗体
英文名称：Rabbit Anti-RNF105 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human RNF105
抗原表位：251-350/2025
SWISS：P53804
Gene ID ：7267
Human Gene ID：7267
E3 ubiquitin-protein ligase that mediates the ubiquitination and subsequent degradation of phosphorylated Akt (AKT1, AKT2 and AKT3) in the nucleus. Acts as a terminal regulator of Akt signaling after activation; its phosphorylation by Akt, which is a prerequisite for ubiquitin ligase activity, suggests the existence of a regulation mechanism required to control Akt levels after activation. Catalyzes the formation of 'Lys-48'-polyubiquitin chains. May play a role in neuronal differentiation inhibition via its interaction with CIT.
Function：E3 ubiquitin-protein ligase that mediates the ubiquitination and subsequent degradation of phosphorylated Akt (AKT1, AKT2 and AKT3) in the nucleus. Acts as a terminal regulator of Akt signaling after activation; its phosphorylation by Akt, which is a prer
Subunit：Interacts (when phosphorylated on Ser-378) with AKT1, AKT2 and AKT3 (when phosphorylated). Interacts with CIT.
Subcellular Location：Nucleus.
Tissue Specificity：Found in all tissues examined.
Post-translational modifications：Phosphorylation on Ser-378 by Akt is required for ubiquitin ligase activity.
Similarity：Contains 1 RING-type zinc finger.
Contains 4 TPR repeats.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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