| Rabbit Anti-CK14+17+42+10 antibody |
| 反应物种（预测） |
Rat |
| 产品应用（已验证） |
WB,IHC |
| 推荐稀释比例 |
WB=1:500-2000,IHC-P=1:100-500, |
| 研究领域 |
肿瘤,细胞生物,免疫学 |
| 标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK14+17+42+10) Polyclonal Antibody, Unconjugated (bs-22150R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Sample:
Uterus (Mouse) Lysate at 40 ug
A431(Human) Cell Lysate at 30 ug
Ear (Mouse) Lysate at 40 ug
Primary: Anti- CK14+17+42+10 (bs-22150R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
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Sample:
Esophageal (Mouse) Lysate at 40 ug
Primary: Anti-CK14+17+42+10 (bs-22150R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
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Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti-CK14+17+42+10 (bs-10196R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
-
Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti-CK14+17+42+10 (bs-22150R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
RRID：RRID
产品名称：Rabbit Anti-CK14+17+42+10 antibody
别名： Cytokeratin 14; CK 14; ck14; Cytokeratin14; Cytokeratin-14; Dowling Meara; ebs3; Epidermolysis bullosa simplex; k14; Keratin 14; Keratin type I cytoskeletal 14; Keratin14; Koebner; Krt 14; krt14; EBS3; EBS4; NFJ; Keratin, type I cytoskeletal 14; Cytokerat
中文名称：细胞角蛋白14抗体
英文名称：Rabbit Anti-CK14+17+42+10 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human CK14+17+42+10
抗原表位：411-472/472
SWISS：P02533
Gene ID ：3861
Human Gene ID：3861
This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq].
Function：The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
Subunit：Heterotetramer of two type I and two type II keratins. keratin-14 associates with keratin-5. Interacts with TRADD and with keratin filaments. Associates with other type I keratins.
Subcellular Location：Cytoplasm. Nucleus. Note=Expressed in both as a filamentous pattern.
Tissue Specificity：Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the ge
Post-translational modifications：A disulfide bond is formed between rather than within filaments and promotes the formation of a keratin filament cage around the nucleus.
DISEASE：Epidermolysis bullosa simplex, Dowling-Meara type (DM-EBS) [MIM:131760]: A severe form of intraepidermal epidermolysis bullosa characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement. Note=Th
Similarity：Belongs to the intermediate filament family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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