Rabbit Anti-Semaphorin 3F antibody |
反应物种(预测) |
Human,Chicken,Dog,Pig,Cow,Rabbit,Sheep |
产品应用(已验证) |
WB |
产品应用(可尝试) |
ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000, |
研究领域 |
发育生物学,神经生物学 |
标签 |
Array |
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Sample:
Hypophysis (Mouse) Lysate at 40 ug
Primary: Anti- Semaphorin 3F (bs-23306R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 88 kD
Observed band size: 88 kD
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Sample:
Lane 1: Mouse Brain Lysates
Lane 2: Rat Brain Lysates
Primary: Anti-Semaphorin 3F (bs-23306R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 88kDa
Observed band size: 88 kDa
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Sample:
Lung (Mouse) Lysate at 40 ug
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-Semaphorin 3F (bs-23306R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 88 kD
Observed band size: 88 kD
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Sample:
Cerebrum(Mouse) Cell Lysate at 40 ug
Primary: Anti-Semaphorin 3F (bs-23306R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 88 kD
Observed band size: 88 kD
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Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-Semaphorin 3F (bs-23306R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 88 kD
Observed band size: 88 kD
RRID:RRID
产品名称:Rabbit Anti-Semaphorin 3F antibody
别名: Semaphorin-3F; Semaphorin3F; Sema III/F; Sema IV; sema3F; SemaIII/F; SemaIV; Semaphorin IV; SemaphorinIV; SEM3F_HUMAN.
中文名称:臂板蛋白3F抗体
英文名称:Rabbit Anti-Semaphorin 3F antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:分泌型蛋白
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human Sema3F
抗原表位:201-300/785
SWISS:Q13275
Gene ID :6405
Human Gene ID:6405
The semaphorins are a family of proteins that are involved in signaling. All the family members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues(Kolodkin et al., 1993 [PubMed 8269517]). Sequence comparisons have grouped the secreted semaphorins into 3 general classes, all of which also have an immunoglobulin domain. The semaphorin III family, consisting of human semaphorin III (SEMA3A; MIM 603961), chicken collapsin, and mouse semaphorins A, D, and E, all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones (Luo et al., 1993 [PubMed 8402908]) through an interaction with a collapsin response mediator protein of relative molecular mass 62K (CRMP62) (Goshima et al., 1995 [PubMed7637782]), a putative homolog of an axonal guidance associated UNC33 gene product (MIM 601168). SEMA3F is a secreted member of the semaphorin III family.
Function:May play a role in cell motility and cell adhesion.
Subunit:Belongs to the semaphorin family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Sema domain.
Subcellular Location:Secreted.
Tissue Specificity:Expressed abundantly but differentially in a variety of neural and nonneural tissues. There is high expression in mammary gland, kidney, fetal brain, and lung and lower expression in heart and liver.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.