| Rabbit Anti-CD14 antibody |
| 反应物种（预测） |
Rat |
| 产品应用（已验证） |
WB,FCM |
| 推荐稀释比例 |
WB=1:500-2000,Flow Cyt=1ug/Test, |
| 研究领域 |
转录调节因子,细胞膜受体,糖尿病, |
| 标签 |
Array |
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Blank control (blue line): Mouse thymus cells (fixed with 70% methanol (Overnight at 4℃)).
Primary Antibody (green line): Rabbit Anti-CD14 antibody (bs-20795R),Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
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Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti-CD14 (bs-20795R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35/40 kD
Observed band size: 42 kD
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Blank control: Raw264.7.
Primary Antibody (green line): Rabbit Anti-CD14 antibody (bs-20795R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID：RRID
产品名称：Rabbit Anti-CD14 antibody
别名： CD14 antigen; CD14 molecule; Lipopolysaccharide receptor; LPSR; Monocyte Differentiation Antigen 14; Monocyte differentiation antigen CD14; Myeloid cell specific leucine rich glycoprotein; CD14_MOUSE; Myeloid cell-specific leucine-rich glycoprotein.
中文名称：内毒素受体抗体
英文名称：Rabbit Anti-CD14 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from mouse CD14
抗原表位：241-300/366
SWISS：P08571
Gene ID ：12475
Human Gene ID：929
The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide, and to viruses. This gene has been identified as a target candidate in the treatment of SARS-CoV-2-infected patients to potentially lessen or inhibit a severe inflammatory response. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Aug 2020]
Function：Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molec
Subunit：Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, MD-2 and TLR4.
Subcellular Location：Cell membrane; Lipid-anchor, GPI-anchor.
Similarity：Contains 11 LRR (leucine-rich) repeats.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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