Rabbit Anti-TRIM38 antibody |
反应物种(预测) |
Human |
产品应用(已验证) |
FCM |
产品应用(可尝试) |
WB,IHC,ICC,IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=2ug/Test,IF=1:100-500,ICC=1:100-500, |
标签 |
Array |
-
Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-TRIM38 antibody (bs-20811R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID:RRID
产品名称:Rabbit Anti-TRIM38 antibody
别名: Gm23;
MGC8946;
RING finger protein 15;
RNF15;
Ro/SSA ribonucleoprotein homolog;
RORET; RP23 480B19.4;
tripartite motif containing 38;
Zinc finger protein RoRet;
A0A6P5R0Q4_MUSCR.
中文名称:TRIM38蛋白抗体
英文名称:Rabbit Anti-TRIM38 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞浆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from mouse TRIM38
抗原表位:301-400/471
SWISS:A0A6P5R0Q4
Gene ID :214158
Human Gene ID:10475
This gene encodes a member of the tripartite motif (TRIM) family. The encoded protein contains a RING-type zinc finger, B box-type zinc finger and SPRY domain. The function of this protein has not been identified. A pseudogene of this gene is located on the long arm of chromosome 4. [provided by RefSeq, Jul 2012]
Function:The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The function of this protein has not been identifi
Tissue Specificity:Ubiquitous.
Similarity:Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.