Rabbit Anti-MetAP1 antibody |
反应物种(预测) |
Human,Chicken,Dog,Cow,Horse,Rabbit |
产品应用(已验证) |
IHC |
产品应用(可尝试) |
ICC,IF,ELISA |
推荐稀释比例 |
Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500, |
研究领域 |
肿瘤,细胞生物,信号转导 |
标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MetAP1) Polyclonal Antibody, Unconjugated (bs-18806R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MetAP1) Polyclonal Antibody, Unconjugated (bs-18806R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID:RRID
产品名称:Rabbit Anti-MetAP1 antibody
别名: Methionyl Aminopeptidase 1;
AMPM1_HUMAN;
DKFZp781C0419;
KIAA0094;
MAP 1;
MAP1A;
MetAP-1;
MetAP1;
metap1;
MetAP1A;
Methionine aminopeptidase 1;
Peptidase M 1.
中文名称:氨基肽酶1抗体
英文名称:Rabbit Anti-MetAP1 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞浆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human MetAP1
抗原表位:51-150/386
SWISS:P53582
Gene ID :23173
Human Gene ID:23173
MetAP-1 is a 394-amino acid protein that is expressed at low levels in all tissues, but is highly expressed in skeletal muscles. The active site of MetAP-1 contains two adjacent divalent metal ions connected by a water molecule or hydroxide ion. The control of cell proliferation in mammalian cells is directly linked and strictly dependent on the evolutionarily highly conserved mechanism that MetAP-1 employs. Eukaryotes contain both MetAP-1 and MetAP-2, whereas prokaryotes possess only the MetAP-1 enzyme. Pyridine-2-carboxylic acid thiazol-2-ylamide (PCAT) forms a scaffold that inhibits the action of MetAP-1, while 1,2,4-triazol is a non-peptide inhibitor of MetAP-1 binding to the active site with the N1 and N2 atoms of the triazole moiety complexing two divalent ions.
Function:Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Required for normal p
Subunit:Associates with the 60S ribosomal subunit of the 80S translational complex.
Subcellular Location:Cytoplasm.
Similarity:Belongs to the peptidase M24A family.
Methionine aminopeptidase type 1 subfamily.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.