Rabbit Anti-C3orf32 antibody |
反应物种(预测) |
Human,Dog,Sheep |
产品应用(已验证) |
WB,IHC |
产品应用(可尝试) |
ICC,IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500, |
研究领域 |
肿瘤,心血管,细胞生物,免疫学,发育生物学,表观遗传学, |
标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (Mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C3orf32) Polyclonal Antibody, Unconjugated (bs-15174R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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Sample:
Lane 1: Testis (Mouse) Lysate at 40 ug
Lane 2: Epididymis (Rat) Lysate at 40 ug
Lane 3: Small intestine (Rat) Lysate at 40 ug
Primary: Anti- C3orf32 (bs-15174R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 40 kD
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Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (chromosome 3 open reading frame 32; C3orf32) Polyclonal Antibody, Unconjugated (bs-15174R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (Rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C3orf32) Polyclonal Antibody, Unconjugated (bs-15174R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
RRID:RRID
产品名称:Rabbit Anti-C3orf32 antibody
别名: chromosome 3 open reading frame 32; fls485; SSU-2; SSUH2; SSUH2 ssu-2 homolog (C. elegans); uncharacterized protein C3orf32 homolog; SSUH2_HUMAN.
中文名称:3号染色体开放阅读框32抗体
英文名称:Rabbit Anti-C3orf32 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞浆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human C3orf32
抗原表位:51-150/353
SWISS:Q9Y2M2
Gene ID :51066
Human Gene ID:51066
C3orf32 (chromosome 3 open reading frame 32), also known as fls485, is a 353 amino acid protein encoded by a gene that maps to human chromosome 3p26.1. Chromosome 3 is made up of approximately 214 million bases encoding over 1,100 genes. Notably, there is a chemokine receptor gene cluster and a variety of human cancer related loci on chromosome 3. Particular regions of the chromosome 3 short arm are deleted in many types of cancer cells. Key tumor suppressing genes on chromosome 3 encode apoptosis mediator RASSF1, cell migration regulator HYAL1 and angiogenesis suppressor SEMA3B. Marfan Syndrome, porphyria, von Hippel-Lindau syndrome, osteogenesis imperfecta and Charcot-Marie-Tooth disease are a few of the numerous genetic diseases associated with chromosome 3.
Subcellular Location:Cytoplasm.
Tissue Specificity:Expressed in enterocytes of small and large intestinal mucosa (at protein level). Expressed in enterocytes, chromaffine and interstitial cells.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.