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Rabbit Anti-CD11c antibody
多克隆  |   SKU:bs-20690R

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货号:bs-3351R
¥1280
订购号:bs-20690R
¥1098.00-2900.00
货期:现货
Rabbit Anti-CD11c antibody
反应物种(预测)

Human,Rat

产品应用(已验证)

WB,FCM

推荐稀释比例

WB=1:500-2000,Flow Cyt=1μg/Test,

研究领域

细胞生物,免疫学,信号转导,干细胞,细胞粘附分子,

标签

Array

  • Blank control: Mouse Spleen(fixed with 2% paraformaldehyde for 10 min at 37℃).
    Primary Antibody:Rabbit Anti-Integrin alpha X /CD11c antibody (bs-20690R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
    Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions;
    Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
  • Sample:
    Raw264.7 (Mouse) Lysate at30 ug
    Primary: Anti-Integrin alpha X /CD11c (bs-20690R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 126 kD
    Observed band size: 135 kD
  • Blank control: Raw264.7.
    Primary Antibody (green line): Rabbit Anti-Integrin alpha X /CD11c antibody (bs-20690R)
    Dilution: 1μg /10^6 cells;
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody : Goat anti-rabbit IgG-AF647
    Dilution: 1μg /test.
    Protocol
    The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

产品信息

RRID:RRID
产品名称:Rabbit Anti-CD11c antibody
别名: Integrin alpha-X; Integrin alpha X; integrin alpha-X precursor; ITGAX; SLEB6; CD11C (p150) alpha polypeptide; complement receptor 4; integrin aX; CD11 antigen-like family member C; leukocyte adhesion receptor p150,95; leukocyte adhesion glycoprotein p150,
中文名称:整合素αX抗体
英文名称:Rabbit Anti-CD11c antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免 疫 原:KLH conjugated synthetic peptide derived from human Integrin alpha X /CD11c
抗原表位:901-1000/1163
抗原细胞定位:Extracellular
SWISS:P20702
Gene ID :3687
Human Gene ID:3687

产品介绍

This gene encodes the integrin alpha X chain protein. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. [provided by RefSeq].
Function:Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.
Subcellular Location:Membrane.
Tissue Specificity:Predominantly expressed in monocytes and granulocytes.
Similarity:Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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