Rabbit Anti-FMNL3 antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

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Rabbit Anti-FMNL3 antibody

多克隆 | SKU：bs-16151R

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货号:bs-3351R

￥1280

50μg

RMB1280

100μg

RMB1980

200μg

RMB2980

支持数据
产品信息
免疫原信息
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订购号:bs-16151R

￥1138.00-2900.00

50ul

100ul

200ul

货期：现货

售后保障

Rabbit Anti-FMNL3 antibody
反应物种（预测）	Mouse,Rat,Dog,Cow
产品应用（已验证）	IHC,FCM
产品应用（可尝试）	WB,ICC,IF,ELISA
推荐稀释比例	WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/test,IF=1:100-500,ICC=1:100-500,
研究领域	细胞生物,免疫学,信号转导,结合蛋白,细胞骨架,
标签	Array

Blank control: Molt4.
Primary Antibody (green line): Rabbit Anti- FMNL3/FITC Conjugated antibody (bs-16151R-FITC)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-FITC .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FMNL3) Polyclonal Antibody, Unconjugated (bs-16151R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

产品信息

RRID：RRID
产品名称：Rabbit Anti-FMNL3 antibody
别名： FHOD3; FMNL3_HUMAN； Formin homology 2 domain containing protein 3; Formin like protein 3; WBP3; WW domain binding protein 3.
中文名称：FMNL3蛋白抗体
英文名称：Rabbit Anti-FMNL3 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated synthetic peptide derived from human FMNL3
抗原表位：951-1028/1028
SWISS：Q8IVF7
Gene ID ：91010
Human Gene ID：91010

产品介绍

The protein encoded by this gene contains a formin homology 2 domain and has high sequence identity to the mouse Wbp3 protein. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
Function：FMNL3 is an actin-binding protein that contains a formin homology 2 domain.
Subunit：Interacts with SRGAP2 (via SH3 domain).
Subcellular Location：Cytoplasm. Note=Enriched in lamellipodia.
Similarity：Belongs to the formin homology family.
Contains 1 DAD (diaphanous autoregulatory) domain.
Contains 1 FH2 (formin homology 2) domain.
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.