| Rabbit Anti-CMKLR1 antibody |
| 反应物种（预测） |
Dog,Sheep |
| 产品应用（已验证） |
WB |
| 产品应用（可尝试） |
IHC,ICC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500, |
| 研究领域 |
肿瘤,细胞生物,染色质和核信号,信号转导,G蛋白偶联受体,表观遗传学,G蛋白信号, |
| 标签 |
Array |
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Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CMKLR1 Polyclonal Antibody, Unconjugated(bs-10183R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Tissue/cell: Human laryngeal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CMKLR1 Polyclonal Antibody, Unconjugated(bs-10183R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Sample:
Lane 1: MCF-7 (Human) Cell Lysate at 30 ug
Lane 2: Huvec (Human) Cell Lysate at 30 ug
Lane 3: HepG2 (Human) Cell Lysate at 30 ug
Primary: Anti-CMKLR1 (bs-10183R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 44 kD
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Sample:
Lane 1: Lung (Mouse) Lysate at 40 ug
Lane 2: Liver (Mouse) Lysate at 40 ug
Primary: Anti-CMKLR1 (bs-10183R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
RRID：RRID
产品名称：Rabbit Anti-CMKLR1 antibody
别名： Chemokine receptor-like 1; CHEMR23; ChemR23; DEZ; Chemokine like receptor 1; G protein coupled receptor ChemR23; G protein coupled receptor DEZ; CML1_HUMAN ; Orphan G protein coupled receptor.
中文名称：趋化样因子受体1抗体
英文名称：Rabbit Anti-CMKLR1 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human CMKLR1
抗原表位：171-280/373
抗原细胞定位：Extracellular
SWISS：Q99788
Gene ID ：1240
Human Gene ID：1240
Chemokine-Like Receptor 1 (CMKLR1) is a serpentine transmembrane G-protein linked receptor. It is an orphan chemoattractant receptor, also known as ChemR23 or DEZ. CMKLR1 is expressed on the surface of monocyte-derived immature dendritic cells and peripheral plamacytoid dendritic (pDC) cells; but not on myeloid dendritic (mDC) cells, monocytes and lymphocytes. The expression of CMKLR1 on pDC is down-regulated after activation. Chemerin (TIG2) is a natural ligand of CMKLR1. The interaction of CMKLR1 with Chemerin induces pDC activation and chemotaxis. It has been shown that CMKLR1 is a coreceptor for several strains of SIV and HIV-1. CMKLR1 is a potential useful marker for distinguishing pDCs and mDCs.
Function：Receptor for the chemoattractant adipokine chemerin/RARRES2 and for the omega-3 fatty acid derived molecule resolvin E1. Interaction with RARRES2 induces activation of intracellular signaling molecules, such as SKY, MAPK1/3 (ERK1/2), MAPK14/P38MAPK and PI
Subcellular Location：Cell membrane; Multi-pass membrane protein.
Tissue Specificity：Prominently expressed in developing osseous and cartilaginous tissue. Also found in adult parathyroid glands. Prominently expressed in developing osseous and cartilaginous tissue. Also found in adult parathyroid glands. Expressed in cardiovascular system,
Similarity：Belongs to the G-protein coupled receptor 1 family.
Database links : UniProtKB/Swiss-Prot: Q99788.2
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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