| Rabbit Anti-Cyclin B1 antibody |
| 反应物种（预测） |
Rat,Cow,Rabbit,Sheep |
| 产品应用（已验证） |
WB |
| 产品应用（可尝试） |
IHC,ICC,IF |
| 推荐稀释比例 |
WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100, |
| 研究领域 |
肿瘤,细胞生物,免疫学,细胞周期蛋白 |
| 标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cyclin B1) Polyclonal Antibody, Unconjugated (bs-23016R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Sample: Jurkat Cell Lysate at 40 ug
Primary: Anti-Cyclin B1(bs-23016R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48kD
Observed band size: 45 kD
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Sample:
Lane 1: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 2: Hela (Human) Cell Lysate at 30 ug
Lane 3: Jurkat (Human) Cell Lysate at 30 ug
Lane 4: U2os (Human) Cell Lysate at 30 ug
Lane 5: K562 (Human) Cell Lysate at 30 ug
Primary: Anti-Cyclin B1 (bs-23016R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55-60 kD
Observed band size: 60 kD
RRID：RRID
产品名称：Rabbit Anti-Cyclin B1 antibody
别名： CCNB 1; CCNB; CCNB1; CCNB1_HUMAN; G2 mitotic specific cyclin B1; G2/mitotic-specific cyclin-B1.
中文名称：周期素B1抗体
英文名称：Rabbit Anti-Cyclin B1 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human Cyclin B1
抗原表位：351-433/433
SWISS：P14635
Gene ID ：891
Human Gene ID：891
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008].
Function：Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Subunit：Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active
Subcellular Location：Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, centrosome.
Post-translational modifications：Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phos
Similarity：Belongs to the cyclin family. Cyclin AB subfamily.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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