Rabbit Anti-ADORA2B antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

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Rabbit Anti-ADORA2B antibody

多克隆 | SKU：bs-10217R

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货号:bs-3351R

￥1280

50μg

RMB1280

100μg

RMB1980

200μg

RMB2980

支持数据
产品信息
免疫原信息
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订购号:bs-10217R

￥1138.00-2900.00

50ul

100ul

200ul

货期：现货

售后保障

Rabbit Anti-ADORA2B antibody
反应物种（预测）	Mouse,Dog,Rabbit
产品应用（已验证）	WB,IHC
产品应用（可尝试）	ICC,IF
推荐稀释比例	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
标签	Array

Tissue/cell: Rat brain; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ADORA2B Polyclonal Antibody, Unconjugated(bs-10217R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Protein: mouse lung lysate;
Primary: rabbit Anti-ADORA2B (bs-10217R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size:37 kD
Observed band size: 35 kD
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ADORA2B Polyclonal Antibody, Unconjugated(bs-10217R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ADORA2B) Polyclonal Antibody, Unconjugated (bs-10217R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sample:
Lane 1: 293T (Human) Cell Lysate at 30 ug
Lane 2: A549 (Human) Cell Lysate at 30 ug
Primary: Anti-DORA2B (bs-10217R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36-41 kD
Observed band size: 40 kD

产品信息

RRID：RRID
产品名称：Rabbit Anti-ADORA2B antibody
别名： Adenosine A2b Receptor; adora 2; adora 2b; ADORA2; ADORA2B; HGNC:264; Netrin 1 receptor; AA2BR_HUMAN.
中文名称：腺苷A2b受体/神经生长因子1受体抗体
英文名称：Rabbit Anti-ADORA2B antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated synthetic peptide derived from human ADORA2B
抗原表位：231-332/332
抗原细胞定位：Cytoplasmic
SWISS：P29274
Gene ID ：136
Human Gene ID：136

产品介绍

This gene encodes an adenosine receptor that is a member of the G protein-coupled receptor superfamily. This integral membrane protein stimulates adenylate cyclase activity in the presence of adenosine. This protein also interacts with netrin-1, which is involved in axon elongation. The gene is located near the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008].
Function：Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Subcellular Location：Cell membrane; Multi-pass membrane protein.
Similarity：Belongs to the G-protein coupled receptor 1 family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.