Rabbit Anti-NFIB antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

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Rabbit Anti-NFIB antibody

多克隆 | SKU：bs-11899R

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货号:bs-3351R

￥1280

50μg

RMB1280

100μg

RMB1980

200μg

RMB2980

支持数据
产品信息
免疫原信息
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订购号:bs-11899R

￥1138.00-2900.00

50ul

100ul

200ul

货期：现货

售后保障

Rabbit Anti-NFIB antibody
反应物种（预测）	Rat,Chicken,Pig,Cow,Rabbit,Sheep
产品应用（已验证）	WB,FCM
产品应用（可尝试）	IHC,ICC,IF,ELISA
推荐稀释比例	WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/Test,IF=1:100-500,ICC=1:100,
研究领域	细胞生物,神经生物学,表观遗传学,
标签	Array

Sample:293T Cell (Human) Lysate at 40 ug
Primary: Anti-NFIB (bs-11899R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 47 kD
Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-NFIB (bs-11899R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 48 kD
Sample:
Lane 1: Hela (Human) Cell Lysate at 30 ug
Lane 2: A431 (Human) Cell Lysate at 30 ug
Lane 3: Lung (Mouse) Lysate at 40 ug
Lane 4: Ovary (Mouse) Lysate at 40 ug
Lane 5: Kidney (Mouse) Lysate at 40 ug
Primary: Anti-NFIB (bs-11899R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 47 kD
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-NFIB antibody (bs-11899R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

产品信息

RRID：RRID
产品名称：Rabbit Anti-NFIB antibody
别名： CCAAT Box Binding Transcription Factor; CCAAT-box-binding transcription factor; CTF; NF-I/B; NF1-B; NF1B; NF1B2; NFI-B; NFI-RED; NFIB; NFIB_HUMAN; NFIB3; Nuclear factor 1 B-type; Nuclear factor 1/B; Nuclear Factor 1B; Nuclear Factor I B; nuclear factor I/
中文名称：核因子1B抗体
英文名称：Rabbit Anti-NFIB antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated synthetic peptide derived from human NFIB/NF1B2
抗原表位：1-100/420
SWISS：O00712
Gene ID ：4781
Human Gene ID：4781

产品介绍

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Function：Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Subunit：Binds DNA as a homodimer.
Subcellular Location：Nucleus.
Similarity：Belongs to the CTF/NF-I family.
Contains 1 CTF/NF-I DNA-binding domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.