| Rabbit Anti-DSPP antibody |
| 反应物种（预测） |
Mouse,Rat |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
ICC,IF |
| 推荐稀释比例 |
WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500, |
| 研究领域 |
细胞生物,发育生物学,信号转导,细胞周期蛋白,结合蛋白,细胞分化,细胞骨架,细胞外基质, |
| 标签 |
Array |
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Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti-DSPP (bs-8557R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47/129 kD
Observed band size: 129 kD
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Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-DSPP Polyclonal Antibody, Unconjugated(bs-8557R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Sample:
Lane 1: Hela (Human) Cell Lysate at 30 ug
Lane 2: SW480 (Human) Cell Lysate at 30 ug
Lane 3: Du145 (Human) Cell Lysate at 30 ug
Primary: Anti-DSPP (bs-8557R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 140 kD
Observed band size: 130 kD
RRID：RRID
产品名称：Rabbit Anti-DSPP antibody
别名： Dentin phosphophoryn; Dentin phosphoprotein; dentin phosphoryn; Dentin sialophosphoprotein; Dentin sialophosphoprotein precursor; Dentin sialoprotein; dentinogenesis imperfecta 1; DFNA39; DGI1; DMP3; DPP; DSP; DSPP_HUMAN; DTDP2.
中文名称：牙本质磷蛋白抗体
英文名称：Rabbit Anti-DSPP antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞外基质,分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human Dentin sialoprotein
抗原表位：101-220/1301
SWISS：Q9NZW4
Gene ID ：1834
Human Gene ID：1834
This gene encodes two principal proteins of the dentin extracellular matrix of the tooth. The preproprotein is secreted by odontoblasts and cleaved into dentin sialoprotein and dentin phosphoprotein. Dentin phosphoprotein is thought to be involved in the biomineralization process of dentin. Mutations in this gene have been associated with dentinogenesis imperfecta-1; in some individuals, dentinogenesis imperfecta occurs in combination with an autosomal dominant form of deafness. Allelic differences due to repeat polymorphisms have been found for this gene. [provided by RefSeq, Jul 2008]
Function：DSP may be an important factor in dentinogenesis. DPP may bind high amount of calcium and facilitate initial mineralization of dentin matrix collagen as well as regulate the size and shape of the crystals.
Subunit：Interacts with FBLN7.
Subcellular Location：Secreted, extracellular space, extracellular matrix.
Tissue Specificity：Expressed in teeth. DPP is synthesized by odontoblast and transiently expressed by pre-ameloblasts.
Post-translational modifications：DSP is glycosylated.
DISEASE：Defects in DSPP are the cause of deafness autosomal dominant type 39 with dentinogenesis imperfecta 1 (DFNA39/DGI1)
[MIM:605594]. Affected individuals present DGI1 associated with early onset progressive sensorineural high-frequency hearing loss.
De
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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