| Rabbit Anti-UCHL1+3 antibody |
| 反应物种（预测） |
Rat,Dog,Pig,Horse,Rabbit,Sheep |
| 产品应用（已验证） |
WB |
| 产品应用（可尝试） |
IHC,ICC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500, |
| 研究领域 |
细胞生物,神经生物学,细胞类型标志物(Neuron marker),泛素, |
| 标签 |
Array |
-
Sample:
Cerebral cortex (Mouse) Lysate at 40 ug
Primary: Anti- UCHL1+3 (bs-11677R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 27 kD
-
Sample:
Hippocampus (Mouse) Lysate at 40 ug
Cerebellum (Mouse) Lysate at 40 ug
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- UCHL1+3 (bs-11677R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 27 kD
-
Sample:
U251(Human) Cell Lysate at 30 ug
Primary: Anti-UCHL1+3 (bs-11677R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 25 kD
-
Sample:
Hippocampus (Mouse) Lysate at 40 ug
Primary: Anti- UCHL1+3 (bs-11677R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 26 kD
RRID：RRID
产品名称：Rabbit Anti-UCHL1+3 antibody
别名： Epididymis luminal protein 117;
Epididymis secretory protein Li 53;
HEL 117;
HEL S 53;
NDGOA;
Neuron cytoplasmic protein 9.5;
OTTHUMP00000218137;
OTTHUMP00000218139;
OTTHUMP00000218140;
OTTHUMP00000218141;
Park 5;
PARK5;
PGP 9.5;
PGP9.5;
PGP
中文名称：泛素硫酯酶L1+3抗体
英文名称：Rabbit Anti-UCHL1+3 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆,细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human UCHL1+3
抗原表位：141-223/223
SWISS：P09936
Gene ID ：7345
Human Gene ID：7345
The two ubiquitin C-terminal hydrolase (UCH) enzymes, UCHL1 and UCHL3, deubiquitinate ubiquitin-protein conjugates and control the cellular balance of ubiquitin. UCHL1 and UCHL3 are both small proteins of ~220 amino acids that share more than 40% amino acid sequence identity. UCHL3 is universally expressed in all tissues, while UCHL1 is expressed exclusively in neuronal tissue, testis and ovary. The activity of UCHL3 is more than 200 fold higher than UCHL1 when a fluorogenic ubiquitin substrate is used. UCHL1 associates with monoubiquitin and UCHL3 binds to Nedd8, ubiquitin-like protein. UCHL1 and UCHL3 play a role in the regulation of neuronal development and spermatogenesis. UCHL1 is involved in the pathogenesis of Parkinson’s disease (PD) and Alzheimer’s disease (AD).
Function：Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free mon
Subunit：Monomer. Homodimer. Interacts with SNCA (By similarity). Interacts with COPS5.
Subcellular Location：Cytoplasm. Endoplasmic reticulum membrane; Lipid-anchor. Note=About 30% of total UCHL1 is associated with membranes in brain.
Tissue Specificity：Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and A
Post-translational modifications：O-glycosylated
DISEASE：Defects in UCHL1 are the cause of Parkinson disease type 5 (PARK5) [MIM:613643]; also known as Parkinson disease autosomal dominant 5. PARK5 is a complex neurodegenerative disorder with manifestations ranging from typical Parkinson disease to dementia wit
Similarity：Belongs to the peptidase C12 family.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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