Rabbit Anti-OSTM1 antibody |
反应物种(预测) |
Pig |
产品应用(已验证) |
WB,FCM |
产品应用(可尝试) |
IHC,IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg/Test,IF=1:50-200, |
标签 |
Array |
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Positive control: mouse Splenocytes(2% Paraformaldehyde-fixed )
Isotype Control Antibody: Rabbit IgG; Dilution: 1μg in 100 μl 1 X PBS containing 0.5% BSA
Secondary Antibody: Goat anti-rabbit IgG-FITC;
Dilution: 1:200 in 1 X PBS containing 0.5% BSA
Primary Antibody :rabbit Anti-OSTM1 (bs-8506R);
Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA
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Sample:
Lane 1: Mouse Spleen tissue lysates
Lane 2: Mouse Lung tissue lysates
Lane 3: Rat Liver tissue lysates
Lane 4: Rat Spleen tissue lysates
Lane 5: Human U87MG cell lysates
Lane 6: Human U251 cell lysates
Lane 7: Human THP-1 cell lysates
Primary: Anti-OSTM1 (bs-8506R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 35 kD
RRID:RRID
产品名称:Rabbit Anti-OSTM1 antibody
别名: GAIP-interacting protein N terminus; GIPN; GL antibody Grey lethal osteopetrosis; HSPC019; OPTB5; Osteopetrosis-associated transmembrane protein 1; Ostm1; OSTM1_HUMAN.
中文名称:骨硬化病相关跨膜蛋白1抗体
英文名称:Rabbit Anti-OSTM1 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human OSTM1
抗原表位:21-120/334
抗原细胞定位:Extracellular
SWISS:Q86WC4
Gene ID :28962
Human Gene ID:28962
OSTM1 (osteopetrosis associated transmembrane protein 1), also known as gl (gray-lethal) or HSPC019, is a 338 amino acid single-pass type I membrane protein that is expressed primarily in osteoclasts and melanocytes as well as brain, kidney and spleen. Bone autosomal recessive osteopetrosis (ARO) is the most severe form of hereditary bone disease whose cellular basis is in the osteoclast and is characterized by abnormally dense bone, due to defective resorption of immature bone. ARO is suggested to be caused by mutations in the OSTM1 gene. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Defects in the OSTM1 gene are also the cause of the spontaneous gl mutant, which is responsible for a coat color defect in mice.
Function:Required for osteoclast and melanocyte maturation and function (By similarity).
Subcellular Location:Membrane; Single-pass type I membrane protein.
DISEASE:Defects in OSTM1 are the cause of osteopetrosis autosomal recessive type 5 (OPTB5) [MIM:259720]; also called infantile malignant osteopetrosis 3. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption o
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.