Rabbit Anti-Uricase antibody |
反应物种(预测) |
Rat,Dog,Pig,Cow,Rabbit |
产品应用(已验证) |
WB,IHC |
产品应用(可尝试) |
IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
研究领域 |
细胞生物,神经生物学,信号转导 |
标签 |
Array |
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Sample:
Lane 1: Urinary bladder (Mouse) Lysate at 40 ug
Primary: Anti-Uricase (bs-6716R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kD
Observed band size: 35 kD
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Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Uricase) Polyclonal Antibody, Unconjugated (bs-6716R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID:AB_11066264
产品名称:Rabbit Anti-Uricase antibody
别名: Urate oxidase; Uox; UOX-2; Uri; Uri2; URIC_MOUSE.
中文名称:尿酸酶抗体
英文名称:Rabbit Anti-Uricase antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞浆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from mouse Uricase
抗原表位:21-120/303
SWISS:P25688
Gene ID :114768
Human Gene ID:114768
Uricase catalyzes the oxidation of uric acid to 5 hydroxyisourate which spontaneously decomposes to form allantoin.
Function:Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.
Subcellular Location:Peroxisome.
Post-translational modifications:Acetylation of Lys-118, Lys-164 and Lys-290 is observed in
liver mitochondria from fasted mice but not from fed mice.
Similarity:Belongs to the uricase family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.