Rabbit Anti-NFAM1 antibody |
反应物种(预测) |
Rat,Pig,Horse |
产品应用(已验证) |
FCM |
产品应用(可尝试) |
IHC,ICC,IF,ELISA |
推荐稀释比例 |
Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg /test,IF=1:100-500,ICC=1:100-500, |
研究领域 |
免疫学,染色质和核信号,信号转导 |
标签 |
Array |
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Blank control:U937.
Primary Antibody (green line): Rabbit Anti-NFAM1 antibody (bs-2680R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Blank control:U937.
Primary Antibody (green line): Rabbit Anti-NFAM1 antibody (bs-2680R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID:AB_10855670
产品名称:Rabbit Anti-NFAM1 antibody
别名: NFAT activation molecule 1 precursor; NFAT activation molecule 1 precursor; Calcinerin/NFAT activating ITAM containing protein; CNAIP; NFAT activation molecule 1; Calcineurin/NFAT-activating ITAM-containing protein; NFAT-activating protein with ITAM motif
中文名称:NFAM1抗体
英文名称:Rabbit Anti-NFAM1 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human NFAM1
抗原表位:81-180/270
抗原细胞定位:Extracellular
SWISS:Q8NET5
Gene ID :150372
Human Gene ID:150372
NFAM1 activates cytokine gene promoters such as the IL13 and TNF-alpha promoters. It contains an immunoreceptor tyrosine-based activation motif (ITAM) and is thought to regulate the signaling and development of B-cells.
Function:May function in immune system as a receptor which activates via the calcineurin/NFAT-signaling pathway the downstream cytokine gene promoters. Activates the transcription of IL-13 and TNF-alpha promoters. May be involved in the regulation of B-cell, but n
Subunit:Interacts with ZAP70 and SYK. No direct interaction with the B-cell antigen receptor (BCR).
Subcellular Location:Cell membrane; Single-pass type I membrane protein. Note=Partially recruited to lipid rafts upon BCR stimulation.
Tissue Specificity:Highly expressed in neutrophils, primary monocytes, mast cells, monocytic cell lines and lymphocytes. Also expressed in spleen B and T cells, and lung. Expressed at low level in non-immune tissue.
Post-translational modifications:N-glycosylated.
Similarity:Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Contains 1 ITAM domain.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.