| Rabbit Anti-Connexin 43 antibody |
| 反应物种（预测） |
Chicken,Dog,Pig,Cow,Rabbit |
| 产品应用（已验证） |
WB,IHC,FCM |
| 产品应用（可尝试） |
ICC,IF |
| 推荐稀释比例 |
WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg/Test,IF=1:100-500,ICC=1:100-500, |
| 研究领域 |
肿瘤,免疫学,转录调节因子,细胞粘附分子, |
| 标签 |
Array |
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Tissue/cell: human cervix carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti- Connexin 43 Polyclonal Antibody, Unconjugated(bs-8987R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Tissue/cell: human brain glioma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti- Connexin 43 Polyclonal Antibody, Unconjugated(bs-8987R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Blank control(blue): HUVEC cells(fixed with 2% paraformaldehyde (10 min) ).
Primary Antibody:Rabbit Anti-Connexin 43 antibody(bs-8987R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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Sample:
Lane 1: Mouse Heart tissue lysates
Lane 2: Rat Heart tissue lysates
Lane 3: Mouse Cerebrum tissue lysates
Lane 4: Rat Cerebrum tissue lysates
Lane 5: Mouse Stomach tissue lysates
Primary: Anti-Connexin 43 (bs-8987R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 48 kD
RRID：RRID
产品名称：Rabbit Anti-Connexin 43 antibody
别名： Connexin 43; connexin43; Connexin43v; Cx 43v; CX43; CX 43; CX-43; DFNB38; Gap junction 43 kDa heart protein;Connexin-43; Gap junction alpha 1 protein; Gap junction protein alpha 1 43kDa (connexin 43); Gap junction protein alpha 1 43kDa; Gap junction prote
中文名称：间隙连接蛋白43抗体
英文名称：Rabbit Anti-Connexin 43 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆,细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human Connexin 43
抗原表位：2-100/382
抗原细胞定位：Extracellular
SWISS：P17302
Gene ID ：2697
Human Gene ID：2697
This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia and heart malformations. [provided by RefSeq].
Function：Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May pl
Subcellular Location：Cell membrane; Multi-pass membrane protein. Cell junction, gap junction.
Tissue Specificity：Expressed in the heart and fetal cochlea.
Post-translational modifications：Phosphorylated at Ser-368 by PRKCG; phosphorylation induces disassembly of gap junction plaques and inhibition of gap junction activity. Phosphorylation at Ser-325, Ser-328 and Ser-330 by CK1 modulates gap junction assembly.
DISEASE：Defects in GJA1 are the cause of autosomal dominant oculodentodigital dysplasia (ODDD) [MIM:164200]; also known as oculodentoosseous dysplasia. ODDD is a highly penetrant syndrome presenting with craniofacial (ocular, nasal, dental) and limb dysmorphisms,
Similarity：Belongs to the connexin family. Alpha-type (group II) subfamily.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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