Rabbit Anti-PLAG1 antibody |
反应物种(预测) |
Rat,Dog,Pig,Cow,Horse |
产品应用(已验证) |
WB |
产品应用(可尝试) |
IHC,IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
研究领域 |
肿瘤,免疫学,信号转导,转录调节因子 |
标签 |
Array |
-
Sample:
Lane 1: Human 293T cell Lysates
Lane 2: Human HUVEC cell Lysates
Lane 3: Human Hela cell Lysates
Lane 4: Human Jurkat cell Lysates
Lane 5: Human U2OS cell Lysates
Primary: Anti-PLAG1 (bs-5969R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56kDa
Observed band size: 56kDa
-
Tissue/cell: mouse embryo liver; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PLAG1 Polyclonal Antibody, Unconjugated(bs-5969R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
-
Sample:
Heart(Mouse) Cell Lysate at 40 ug
Primary: Anti-PLAG1 (bs-5969R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
RRID:AB_11073816
产品名称:Rabbit Anti-PLAG1 antibody
别名: FGFR1/PLAG1 fusion variant 3; Pleiomorphic adenoma gene 1 protein; PSA; SGPA; COL1A2/PLAG1 fusion; Plag1; PLAG1_HUMAN; Pleiomorphic adenoma gene 1; Zinc finger protein PLAG1; ZNF912.
中文名称:多型性腺瘤基因1蛋白抗体
英文名称:Rabbit Anti-PLAG1 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞核
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human PLAG1
抗原表位:21-120/500
SWISS:Q6DJT9
Gene ID :5324
Human Gene ID:5324
Pleomorphic adenoma gene 1 encodes a zinc finger protein with 2 putative nuclear localization signals. PLAG1, which is developmentally regulated, has been shown to be consistently rearranged in pleomorphic adenomas of the salivary glands. PLAG1 is activated by the reciprocal chromosomal translocations involving 8q12 in a subset of salivary gland pleomorphic adenomas. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function:Transcription factor whose activation results in up-regulation of target genes, such as IGFII, leading to uncontrolled cell proliferation: when overexpressed in cultured cells, higher proliferation rate and transformation are observed. Other target genes
Subunit:Interacts with KPNA2, which escorts protein to the nucleus via interaction with nuclear localization signal. Interacts with E3 SUMO-protein ligase PIAS1, PIAS2 and PIAS4.
Subcellular Location:Nucleus. Strong nucleolar localization when sumoylation is inhibited.
Tissue Specificity:Expressed in fetal tissues such as lung, liver and kidney. Not detected or weak detection in normal adult tissues, but highly expressed in salivary gland with benign or malignant pleiomorphic adenomas with or without 8q12 abberations, with preferential oc
Post-translational modifications:Sumoylated by SUMO1; which inhibits transcriptional activity, but does not affect nuclear localization. Blockers of sumoylation pathway such as SENP3 and inactive UBE2I increases transcriptional capacity. Sumoylation is increased in the presence of PIAS1.
DISEASE:Note=A chromosomal aberration involving PLAG1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with constituvely expressed beta-catenin/CTNNB1. Fusion occurs in
Similarity:Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 7 C2H2-type zinc fingers.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.