Rabbit Anti-GNB1 antibody |
反应物种(预测) |
Human,Chicken,Dog,Pig,Cow,Horse |
产品应用(已验证) |
WB,IHC |
产品应用(可尝试) |
IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
研究领域 |
细胞生物,免疫学,神经生物学 |
标签 |
Array |
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Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-GNB1 (bs-0348R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD
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Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GNB1) Polyclonal Antibody, Unconjugated (bs-0348R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-G protein beta subunit GI Polyclonal Antibody, Unconjugated(bs-0348R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-G protein beta subunit GI Polyclonal Antibody, Unconjugated(bs-0348R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
RRID:AB_10881433
产品名称:Rabbit Anti-GNB1 antibody
别名: G protein beta subunit GI/GS/GT; guanine nucleotide binding protein (G protein), beta polypeptide 1; guanine nucleotide-binding protein G; GBB1_HUMAN.
中文名称:G蛋白/鸟苷酸结合蛋白抗体
英文名称:Rabbit Anti-GNB1 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞浆,细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human G protein beta subunit GI
抗原表位:101-200/341
SWISS:P62879
Gene ID :2782
Human Gene ID:2782
G protein beta subunit GI/GS/GT (guanine nucleotide-binding protein G). WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.
Function:Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effecto
Subunit:G proteins are composed of 3 units, alpha, beta and gamma. Interacts with ARHGEF18 and RASD2. The heterodimer formed by GNB1 and GNG2 interacts with PTH1R (via C-terminus).
Post-translational modifications:Phosphorylation at His-266 by NDKB contributes to G protein activation by increasing the high energetic phosphate transfer onto GDP.
Similarity:Belongs to the WD repeat G protein beta family.
Contains 7 WD repeats.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.