Rabbit Anti-GLUT2 antibody |
反应物种(预测) |
Horse |
产品应用(已验证) |
WB,IHC,FCM |
产品应用(可尝试) |
IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg/Test,IF=1:100-500, |
标签 |
Array |
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Sample:
Lane 1: Liver (Mouse) Lysate at 40 ug
Lane 2: Kidney (Mouse) Lysate at 40 ug
Lane 3: Pancreas (Mouse) Lysate at 40 ug
Primary: Anti-GLUT2 (bs-10379R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57/45 kD
Observed band size: 47 kD
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Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-GLUT2 (bs-10379R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD
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Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-GLUT2 (bs-10379R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD
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Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GLUT2 Polyclonal Antibody, Unconjugated(bs-10379R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Blank control (blue line): Hep G2(fixed with 70% ethanol Overnight at 4℃).
Primary Antibody (green line): Rabbit Anti-GLUT2 antibody (bs-10379R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
RRID:RRID
产品名称:Rabbit Anti-GLUT2 antibody
别名: liver; Glucose Transporter 2; Glucose Transporter GLUT2; Glucose transporter type 2; Glucose transporter type 2 liver; GLUT-2; GLUT2; GLUT 2; GTR2_HUMAN; SLC2A2; Solute carrier family 2 (facilitated glucose transporter) member 2; Solute carrier family 2 f
中文名称:葡萄糖转运蛋白2抗体
英文名称:Rabbit Anti-GLUT2 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human GLUT2
抗原表位:1-100/524
抗原细胞定位:Extracellular
SWISS:P11168
Gene ID :6514
Human Gene ID:6514
Glucose transporter 2 isoform is an integral plasma membrane glycoprotein of the liver, islet beta cells, intestine, and kidney epithelium. It mediates facilitated bidirectional glucose transport. Because of its low affinity for glucose, it has been suggested as a glucose sensor. [provided by RefSeq, Jul 2008].
Function:Facilitative glucose transporter. This isoform likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism o
Subcellular Location:Membrane; Multi-pass membrane protein.
Tissue Specificity:Liver, insulin-producing beta cell, small intestine and kidney.
Post-translational modifications:N-glycosylated; required for stability and retention at the cell surface of pancreatic beta cells.
DISEASE:Defects in SLC2A2 are the cause of Fanconi-Bickel syndrome (FBS) [MIM:227810]. FBS is a rare, well-defined clinical entity, inherited in an autosomal recessive mode and characterized by hepatorenal glycogen accumulation, proximal renal tubular dysfunction
Similarity:Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.