Rabbit Anti-RNF216 antibody |
反应物种(预测) |
Mouse,Rat,Dog,Horse |
产品应用(已验证) |
IHC |
产品应用(可尝试) |
IF,ELISA |
推荐稀释比例 |
Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200, |
研究领域 |
细胞生物,免疫学,信号转导 |
标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF216) Polyclonal Antibody, Unconjugated (bs-9154R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID:RRID
产品名称:Rabbit Anti-RNF216 antibody
别名: E3 ubiquitin ligase TRIAD3; TRIAD3; ring finger protein 216; Triad domain-containing protein 3; U7I1; UBCE7IP1; Ubiquitin conjugating enzyme 7 interacting protein 1; UiP83; ZIN; Zinc finger protein inhibiting NF kappa B; RN216_HUMAN.
中文名称:锌指蛋白抑制NFκB蛋白抗体
英文名称:Rabbit Anti-RNF216 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞浆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human TRIAD3
抗原表位:301-400/866
SWISS:Q9NWF9
Gene ID :54476
Human Gene ID:54476
The TRIAD3 gene encodes a cytoplasmic protein which specifically colocalizes and interacts with the serine/threonine protein kinase, receptor-interacting protein (RIP). Zinc finger domains of the encoded protein are required for its interaction with RIP and for inhibition of TNF- and IL1-induced NF-kappa B activation pathways. The encoded protein may also function as an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes and transfers it to substrates. Several alternatively spliced transcript variants have been described for this locus but the full-length natures of only some are known.
Function:Isoform 1 acts as an E3 ubiquitin ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Promotes degradation of TRAF3, TLR4 and TLR9. Contributes
Subunit:nteracts with UBE2L3 and to some extent with UBE2L6. Interacts with TRAF3, TLR3, TLR4, TLR5 and TLR9. Isoform 3/ZIN binds RIPK1 and HIV VIF.
Subcellular Location:Cytoplasmic
Tissue Specificity:Ubiquitous, with the highest levels of expression in testis and peripheral blood leukocytes.
Similarity:Contains 1 IBR-type zinc finger.
Contains 2 RING-type zinc fingers.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.