Rabbit Anti-CHST7 antibody |
反应物种(预测) |
Human,Rat,Dog,Pig,Cow,Rabbit,Sheep |
产品应用(已验证) |
WB,IHC |
产品应用(可尝试) |
ICC,IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500, |
研究领域 |
细胞生物,信号转导,新陈代谢, |
标签 |
Array |
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Sample:
Kidney (Mouse) Lysate at 40 ug
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- CHST7 (bs-13968R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CHST7) Polyclonal Antibody, Unconjugated (bs-13968R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Sample:
Ovary (Mouse) Lysate at 40 ug
Primary: Anti- CHST7 (bs-13968R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD
RRID:RRID
产品名称:Rabbit Anti-CHST7 antibody
别名: 6 O sulfotransferase 5;
C6ST 2;
C6ST2;
Carbohydrate sulfotransferase 7;
Chondroitin 6 sulfotransferase 2;
CHST 7; CHST7_HUMAN;
CHST7 carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 7;
Galactose/N acetylglucosamine/N acetylglucosamine 6 O su
中文名称:碳水化合物磺基转移酶7抗体
英文名称:Rabbit Anti-CHST7 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞浆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human CHST7
抗原表位:101-200/486
SWISS:Q9NS84
Gene ID :56548
Human Gene ID:56548
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus reducing the number of supercoils and altering the topology of DNA. This enzyme forms a complex with BLM which functions in the regulation of recombination in somatic cells. [provided by RefSeq, Jul 2008]
Function:CHST7 belongs to the sulfotransferase family. Sulfotransferases generate sulfated glycosaminoglycan (GAG) moities during chondroitin sulfate biosynthesis. They create considerable structural diversity among chondroitin sulfates by transferring sulfate wit
Subcellular Location:Golgi apparatus membrane; Single-pass type II membrane protein
Tissue Specificity:Widely expressed. Highly expressed in heart, spleen, liver and ovary. Expressed at lower level in brain, placenta, thyroid, spinal cord and peripheral blood leukocytes. Not expressed in adult skin.
Similarity:Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.