| Rabbit Anti-CWC22 antibody |
| 反应物种（预测） |
Human,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep |
| 产品应用（已验证） |
IHC |
| 产品应用（可尝试） |
ICC,IF,ELISA |
| 推荐稀释比例 |
Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500, |
| 研究领域 |
细胞生物,转录调节因子,表观遗传学, |
| 标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CWC22) Polyclonal Antibody, Unconjugated (bs-11464R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (mouse lung tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CWC22) Polyclonal Antibody, Unconjugated (bs-11464R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CWC22) Polyclonal Antibody, Unconjugated (bs-11464R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CWC22) Polyclonal Antibody, Unconjugated (bs-11464R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID：RRID
产品名称：Rabbit Anti-CWC22 antibody
别名： CWC22; CWC22 spliceosome associated protein homolog (S. cerevisiae); CWC22 spliceosome associated protein homolog; CWC22_HUMAN; EIF4GL; fSAPb; Functional spliceosome associated protein b; KIAA1604; NCM; Nucampholin homolog; Pre-mRNA-splicing factor CWC22
中文名称：CWC22蛋白抗体
英文名称：Rabbit Anti-CWC22 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human CWC22
抗原表位：101-200/908
SWISS：Q9HCG8
Gene ID ：57703
Human Gene ID：57703
Spliceosomes are multi-protein complexes that are composed of snRNPs (small nuclear ribonucleoproteins) and a variety of associated protein factors, all of which work in concert to regulate the splicing of pre-mRNA, a critical step in the post-transcriptional regulation of gene expression. CWC22 (CWC22 spliceosome-associated protein), also known as NCM, fSAPb or EIF4GL, is a 908 amino acid nuclear protein and component of the spliceosome C complex. CWC22 is associated with the spliceosome prior to catalytic steps and remains associated throughout the reaction. Belonging to the CWC22 family, CW22 contains one MI domain and a MIF4G domain. The gene encoding CWC22 maps to human chromosome 2q31.3. As the second largest human chromosome, chromosome 2 makes up approximately 8% of the human genome and contains 237 million bases encoding over 1,400 genes.
Function：May be involved in pre-mRNA splicing.
Subunit：Component of the spliceosome C complex.
Subcellular Location：Nucleus.
Similarity：Belongs to the CWC22 family.
Contains 1 MI domain.
Contains 1 MIF4G domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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