Rabbit Anti-ANKLE2 antibody |
反应物种(预测) |
Rat,Cow,Horse,Rabbit,Sheep |
产品应用(已验证) |
WB |
产品应用(可尝试) |
IHC,IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200, |
研究领域 |
细胞生物,免疫学 |
标签 |
Array |
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Sample:
HepG2(Human) Cell Lysate at 30 ug
Molt-4Human) Cell Lysate at 30 ug
HL60(Human) Cell Lysate at 30 ug
A431(Human) Cell Lysate at 30 ug
A549(Human) Cell Lysate at 30 ug
Cerebrum (Mouse) Lysate at 40 ug
Stomach (Mouse) Lysate at 40 ug
Lung (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
Heart (Mouse) Lysate at 40 ug
Primary: Anti-ANKLE2 (bs-9743R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 117 kD
Observed band size: 117 kD
RRID:RRID
产品名称:Rabbit Anti-ANKLE2 antibody
别名: ANKL2_HUMAN; ANKLE 2; ANKLE2; ankyrin repeat and LEM domain containing 2; Ankyrin repeat and LEM domain-containing protein 2; LEM domain containing 7; LEMD 7; LEMD7.
中文名称:ANKLE2蛋白抗体
英文名称:Rabbit Anti-ANKLE2 antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞浆,细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human ANKLE2
抗原表位:251-350/938
SWISS:Q86XL3
Gene ID :23141
Human Gene ID:23141
Ankyrins are membrane adaptor molecules that play important roles in coupling integral membrane proteins to the spectrin-based cytoskeleton network. Mutations of ankyrin genes lead to severe genetic diseases such as fatal cardiac arrhythmias and hereditary spherocytosis. ANKLE2 (ankyrin repeat and LEM domain containing 2), also known as LEMD7, is a 938 amino acid single-pass membrane protein containing an ANK repeat and a LEM domain. Exsiting as two isoforms produced by alternative splicing events, the gene encoding ANKLE2 maps to human chromosome 12, which encodes over 1,100 genes and comprises approximately 4.5% of the human genome. Chromosome 12 is associated with a variety of diseases and afflictions, including hypochondrogenesis, achondrogenesis, Kniest dysplasia, Noonan syndrome and trisomy 12p, which causes facial developmental defects and seizure disorders.
Function:Involved in mitotic nuclear envelope reassembly by promoting dephosphorylation of BAF/BANF1 during mitotic exit. Coordinates the control of BAF/BANF1 dephosphorylation by inhibiting VRK1 kinase and promoting dephosphorylation of BAF/BANF1 by protein phosp
Subunit:Interacts with BAF/BANF1. Interacts with protein phosphatase 2A (PP2A) components PPP2C (PPP2CA or PPP2CB) and PPP2R1A.
Subcellular Location:Endoplasmic reticulum membrane; Single-pass type III membrane protein.
Similarity:Belongs to the ANKLE2 family.
Contains 1 ANK repeat.
Contains 1 LEM domain.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.