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Rabbit Anti-eIF3A antibody
多克隆  |   SKU:bs-3840R

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货号:bs-3351R
¥1280
订购号:bs-3840R
¥1138.00-2900.00
货期:现货
Rabbit Anti-eIF3A antibody
反应物种(预测)

Rat,Dog,Pig,Cow,Horse,Rabbit

产品应用(已验证)

WB

产品应用(可尝试)

IHC,IF,ELISA

推荐稀释比例

WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,

研究领域

肿瘤,免疫学,信号转导,转录调节因子,表观遗传学,

标签

Array

  • Sample:
    Testis (Mouse) Lysate at 40 ug
    Hela(Human) Cell Lysate at 30 ug
    Primary: Anti- eIF3A (bs-3840R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 166 kD
    Observed band size: 166 kD
  • Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (eIF3A) Polyclonal Antibody, Unconjugated (bs-3840R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

产品信息

RRID:AB_10857343
产品名称:Rabbit Anti-eIF3A antibody
别名: centrosomin homolog; cytoplasmic protein p167; eIF 3 theta; eIF-3-theta; EIF3; eIF3 p167; eIF3 p170; eIF3 p180; EIF3 p180 subunit; eIF3 p185; eIF3 theta; eIF3a; EIF3S10; eukaryotic translation initiation factor 3 subunit 10 (theta 150 170kD); eukaryotic t
中文名称:真核翻译起始因子3A抗体
英文名称:Rabbit Anti-eIF3A antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞浆
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免 疫 原:KLH conjugated synthetic peptide derived from human eIF3A
抗原表位:801-900/1382
SWISS:Q14152
Gene ID :8661
Human Gene ID:8661

产品介绍

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Subcellular Location:Cytoplasm.
Similarity:Belongs to the eIF-3 subunit A family.
Contains 1 PCI domain.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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