| Rabbit Anti-Galectin 9 antibody |
| 反应物种（预测） |
Rat,Pig,Cow |
| 产品应用（已验证） |
WB,FCM |
| 产品应用（可尝试） |
ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,Flow Cyt=1ug/Test, |
| 研究领域 |
细胞生物,细胞凋亡 |
| 标签 |
Array |
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Sample:
NIH/3T3 Cell Lysate at 30 ug
Primary: Anti-Galectin 9 (bs-1699R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35-39 kD
Observed band size: 35kD
-
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-Galectin 9 (bs-1699R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35-39 kD
Observed band size: 39 kD
-
Blank control:Molt4.
Primary Antibody (green line): Rabbit Anti-Galectin 9 antibody (bs-1699R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID：AB_10857547
产品名称：Rabbit Anti-Galectin 9 antibody
别名： LGALS9; 36 kDa beta-galactoside-binding lectin; Ecalectin; Gal-9; Galectin-9; galectin9; HOM HD 21; HOMHD21; HUAT; Lectin galactoside binding soluble 9; LEG9_HUMAN; LGAL S9; LGALS 9; LGALS9A; MGC117375; MGC125973; MGC125974; Tumor antigen HOM-HD-21; UAT;
中文名称：半乳糖凝集素9抗体
英文名称：Rabbit Anti-Galectin 9 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆,分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from mouse Galectin
抗原表位：51-150/353
SWISS：O00182
Gene ID ：3965
Human Gene ID：3965
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin 9 is an S-type lectin and an eosinophil chemoattractant (ECA) produced by antigen-activated T cells. Galectin 9 is involved in apoptosis, is thought to be a prognostic factor for breast cancer and is also overexpressed in Hodgkin's disease tissue. The protein has N- and C- terminal carbohydrate-binding domains connected by a link peptide. Multiple alternatively spliced transcript variants have been found for this gene.
Subunit：Contains 2 galectin domains. Homodimer.
Subcellular Location：Cytoplasm. Secreted. Note=May also be secreted by a non-classical secretory pathway.
Tissue Specificity：Accentuated expression in liver and thymus of embryo, detected in embryonic heart, brain, lung, liver, and kidney. Highly expressed in adult thymus, small intestine, and liver, and to a lesser extent in lung, kidney, spleen, cardiac, and skeletal muscle.
Similarity：Contains 2 galectin domains.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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