| Rabbit Anti-POLK antibody |
| 反应物种（预测） |
Mouse,Rat,Dog,Pig,Cow,Horse,Sheep |
| 产品应用（已验证） |
FCM |
| 产品应用（可尝试） |
WB,IHC,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/Test, |
| 研究领域 |
细胞生物,表观遗传学, |
| 标签 |
Array |
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Blank control (Black line): Molt4 (Black).
Primary Antibody (green line): Rabbit Anti-DNA Polymerase Kappa-POLK antibody (bs-13019R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Blank control:Hela.
Primary Antibody (green line): Rabbit Anti-DNA Polymerase Kappa/POLK antibody (bs-13019R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID：RRID
产品名称：Rabbit Anti-POLK antibody
别名： DINP; POLQ; DINB1; DNA polymerase kappa; DINB protein; POLK_HUMAN.
中文名称：DNA聚合酶κ/DNA pol κ抗体
英文名称：Rabbit Anti-POLK antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human POLK
抗原表位：1-100/870
SWISS：Q9UBT6
Gene ID ：51426
Human Gene ID：51426
This gene encodes a member of the DNA polymerase type-Y family of proteins. The encoded protein is a specialized DNA polymerase that catalyzes translesion DNA synthesis, which allows DNA replication in the presence of DNA lesions. Human cell lines lacking a functional copy of this gene exhibit impaired genome integrity and enhanced susceptibility to oxidative damage. Mutations in this gene that impair enzyme activity may be associated with prostate cancer in human patients. [provided by RefSeq, Sep 2016]
Function：Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Ins
Subunit：Interacts with REV1 (By similarity). Interacts with PCNA.
Subcellular Location：Nucleus. Accumulates at replication forks after DNA damage.
Tissue Specificity：Detected at low levels in testis, spleen, prostate and ovary. Detected at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.
Similarity：Belongs to the DNA polymerase type-Y family.
Contains 1 umuC domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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