| Rabbit Anti-Engrailed 1 antibody |
| 反应物种（预测） |
Chicken,Dog,Pig,Cow,Horse,Sheep |
| 产品应用（已验证） |
WB,FCM |
| 产品应用（可尝试） |
IHC,ICC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg/Test,IF=1:100-500,ICC=1:100-500, |
| 研究领域 |
细胞生物,发育生物学,神经生物学,信号转导,转录调节因子,表观遗传学, |
| 标签 |
Array |
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Blank control(blue): Raji Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
Primary Antibody: Rabbit Anti-Engrailed 1/AF647 Conjugated antibody (bs-11744R-AF647), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
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Sample:
Lane 1: Muscle (Mouse) Lysate at 40 ug
Lane 2: Cerebrum (Mouse) Lysate at 40 ug
Lane 3: Muscle (Rat) Lysate at 40 ug
Lane 4: Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-Engrailed 1 (bs-11744R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43/52 kD
Observed band size: 50 kD
RRID：RRID
产品名称：Rabbit Anti-Engrailed 1 antibody
别名： EN1 / Engrailed 1; Engrailed1; En 1; EN1; EN-1; Engrailed; Engrailed homeobox 1; Engrailed homolog 1; HME1_HUMAN; Homeobox protein en-1; Homeobox protein engrailed 1; Homeobox protein engrailed-1; Homeobox protein engrailed-1; Hu-En-1; Mo-en.1.
中文名称：同源盒蛋白转录因子EN1抗体
英文名称：Rabbit Anti-Engrailed 1 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human EN1/Engrailed 1
抗原表位：351-420/392
SWISS：Q05925
Gene ID ：2019
Human Gene ID：2019
The engrailed-1 gene, EN1, a murine homolog of the Drosophila homeobox gene engrailed (EN), is required for midbrain and cerebellum development and dorsal/ventral patterning of the limbs as well as apical ectodermal ridge formation. In Drosophila, the EN gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Human EN-1 and EN-2 are homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. Different mutations in the mouse homo-logs, EN-1 and EN-2, produce different developmental defects that frequently are lethal. EN-1 is highly expressed by essentially all dopaminergic neurons in the substantia nigra and ventral tegmentum. EN-1 and EN-2 regulate expres-sion of a-synuclein, a gene that is genetically linked to Parkinson’s disease.
Subcellular Location：Nucleus.
Similarity：Belongs to the engrailed homeobox family.
Contains 1 homeobox DNA-binding domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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