Rabbit Anti-CH25H antibody |
反应物种(预测) |
Mouse,Rat,Pig,Rabbit |
产品应用(已验证) |
WB |
推荐稀释比例 |
WB=1:500-2000, |
研究领域 |
肿瘤,细胞生物,信号转导,激酶和磷酸酶,新陈代谢, |
标签 |
Array |
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Sample:
Lane 1: Human U-87 MG cell Lysates
Primary: Anti-CH25H (bs-6480R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32kDa
Observed band size: 32kDa
RRID:RRID
产品名称:Rabbit Anti-CH25H antibody
别名: C25H; Cholesterol 25 hydroxylase; Cholesterol 25-hydroxylase; Cholesterol 25 monooxygenase; EC 1.14.99.38; EC=1.14.99.38; h25OH; CH25H_HUMAN.
中文名称:胆固醇25羟化酶抗体
英文名称:Rabbit Anti-CH25H antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞浆,细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human CH25H
抗原表位:1-100/272
SWISS:O95992
Gene ID :9023
Human Gene ID:9023
CH25H (Cholesterol 25-hydroxylase), also known as h25OH, is a 272 amino acid endoplasmic membrane protein that belongs to the sterol desaturase family. CH25H contains clusters of histidine residues essential for catalytic activity and is involved in cholesterol and lipid metabolism. CH25H catalyzes the formation of 25-hydroxycholesterol from cholesterol leading to the repression of cholesterol biosynthetic enzymes. CH25H regulates lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. CH25H utilizes diiron cofactors to catalyze the hydroxylation of hydrophobic substrates.
Function:CH25H is involved in cholesterol and lipid metabolism and catalyses the formation of 25-hydroxycholesterol from cholesterol, leading to repression of cholesterol biosynthetic enzymes. It may play an important role in regulating lipid metabolism by synthes
Subcellular Location:Endoplasmic reticulum membrane
Post-translational modifications:N-glycosylated.
Similarity:Belongs to the sterol desaturase family.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.