| Rabbit Anti-Phospho-MYPT1 (Thr696) antibody |
| 反应物种（预测） |
Mouse,Rat,Chicken,Dog,Cow,Horse,Rabbit |
| 产品应用（已验证） |
ICC,FCM |
| 产品应用（可尝试） |
WB,IHC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/Test,IF=1:100-500,ICC=1:100, |
| 研究领域 |
肿瘤,免疫学,信号转导,细胞凋亡,转录调节因子 |
| 标签 |
Array |
RRID：RRID
产品名称：Rabbit Anti-Phospho-MYPT1 (Thr696) antibody
别名： Myosin Phosphatase (phospho T696); Myosin Phosphatase (phospho Thr696); p-MYPT1(Thr696); Myosin Phosphatase; M130; MBS; MGC133042; Myosin phosphatase target subunit 1; Myosin phosphatase targeting subunit 1; MYPT 1; MYPT1; PPP1R12A; Protein phosphatase 1
中文名称：磷酸化肌球蛋白磷酸酶抗体
英文名称：Rabbit Anti-Phospho-MYPT1 (Thr696) antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthesised phosphopeptide derived from human MYPT1 around the phosphorylation site of Thr696
抗原表位：RS(p-T)QG
SWISS：O14974
Gene ID ：4659
Human Gene ID：4659
Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in non muscle cells. The guanosine triphosphate (GTP) bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho associated kinase (Rho kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Therefore Rho appears to inhibit myosin phosphatase through the action of Rho kinase.
Function：Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity.
Subunit：Interacts (when phosphorylated at Ser-445, Ser-472 and Ser-910) with 14-3-3. Interacts with ROCK1 and ROCK2. Interacts with isoform 1 and isoform 2 of ZIPK/DAPK3. Interacts with RAF1. Interacts with HIF1AN.
Subcellular Location：Cytoplasm. Note=Along actomyosin filaments and stress fibers.
Tissue Specificity：Expressed in striated muscles, specifically in type 2a fibers (at protein level).
Post-translational modifications：Phosphorylated by CIT (Rho-associated kinase). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR. In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation
Similarity：Contains 6 ANK repeats.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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