Rabbit Anti-IMPDH1 antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

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Rabbit Anti-IMPDH1 antibody

多克隆 | SKU：bs-6256R

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货号:bs-3351R

￥1280

50μg

RMB1280

100μg

RMB1980

200μg

RMB2980

支持数据
产品信息
免疫原信息
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订购号:bs-6256R

￥1138.00-2900.00

50ul

100ul

200ul

货期：现货

售后保障

Rabbit Anti-IMPDH1 antibody
反应物种（预测）	Dog,Pig,Cow,Rabbit,GuineaPig
产品应用（已验证）	WB,IHC,FCM
产品应用（可尝试）	IF,ELISA
推荐稀释比例	WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1ug/Test,IF=1:100-500,
研究领域	肿瘤,细胞生物,免疫学,染色质和核信号,信号转导
标签	Array

Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IMPDH1) Polyclonal Antibody, Unconjugated (bs-6256R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IMPDH1) Polyclonal Antibody, Unconjugated (bs-6256R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-IMPDH1 (bs-6256R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Sample:
Jurkat(Human) Cell Lysate at 30 ug
K562(Human) cell Lysate at 30 ug
NIH/3T3(Mouse) Cell Lysate at 30 ug
Primary: Anti-IMPDH1 (bs-6256R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Blank control:Hela.
Primary Antibody (green line): Rabbit Anti-IMPDH1 antibody (bs-6256R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

产品信息

RRID：AB_11086187
产品名称：Rabbit Anti-IMPDH1 antibody
别名： IMDH1_HUMAN; IMP (inosine monophosphate) dehydrogenase 1; IMP dehydrogenase 1; IMPD 1; IMPD; IMPD1; IMPDH 1; IMPDH I; IMPDH-I; Impdh1; Inosine 5' monophosphate dehydrogenase 1; Inosine monophosphate dehydrogenase 1; Inosine-5''-monophosphate dehydrogenase
中文名称：肌苷单磷酸脱氢酶1抗体
英文名称：Rabbit Anti-IMPDH1 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated synthetic peptide derived from human IMPDH1
抗原表位：201-300/514
SWISS：P20839
Gene ID ：3614
Human Gene ID：3614

产品介绍

The protein encoded by this gene acts as a homotetramer to regulate cell growth. The encoded protein is an enzyme that catalyzes the synthesis of xanthine monophosphate (XMP) from inosine-5'-monophosphate (IMP). This is the rate-limiting step in the de novo synthesis of guanine nucleotides. Defects in this gene are a cause of retinitis pigmentosa type 10 (RP10). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008].
Function：Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Co
Subunit：Homotetramer.
Subcellular Location：Cytoplasm. Nucleus.
Tissue Specificity：IMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor.
DISEASE：Retinitis pigmentosa 10 (RP10) [MIM:180105]: A retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cell
Similarity：Belongs to the IMPDH/GMPR family.
Contains 2 CBS domains.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.