| Rabbit Anti-BCMA antibody |
| 反应物种（预测） |
Mouse,Rat |
| 产品应用（已验证） |
FCM |
| 产品应用（可尝试） |
ELISA |
| 推荐稀释比例 |
Elisa=1:5000-10000,Flow Cyt=1μg/Test, |
| 研究领域 |
肿瘤,细胞生物,免疫学,转录调节因子,B-淋巴细胞, |
| 标签 |
Array |
-
Black line : Positive blank control (Raji); Negative blank control (HL60）
Green line : Primary Antibody (Rabbit Anti-BCMA antibody (bs-3850R) )
Orange line：Isotype Control Antibody (Rabbit IgG) .
Blue line : Secondary Antibody (Goat anti-rabbit IgG-PE)/(Goat anti-rabbit IgG-AF488)
Raji(Positive）and HL60（Negative control）cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with BCMA Antibody(bs-3850R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
-
Blank control: Raji (fixed with 2% paraformaldehyde (10 min),then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody: Rabbit Anti-BCMA antibody(bs-3850R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
RRID：AB_10856548
产品名称：Rabbit Anti-BCMA antibody
别名： B cell maturation antigen; B Cell Maturation Factor; B cell maturation protein; BCM; TNFRSF17; CD269; CD269 antigen; Tumor Necrosis Factor Receptor Member 17; Tumor necrosis factor receptor superfamily member 17; TNR17_HUMAN.
中文名称：B淋巴细胞成熟因子（CD269）抗体
英文名称：Rabbit Anti-BCMA antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human BCMA
抗原表位：12-110/184
抗原细胞定位：Extracellular
SWISS：Q02223
Gene ID ：608
Human Gene ID：608
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13B/TALL-1/BAFF), and to lead to NF-kappaB and MAPK8/JNK activation. This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation. [provided by RefSeq]
Function：Receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK.
Subunit：Associates with TRAF1, TRAF2, TRAF3, TRAF5 and TRAF6.
Subcellular Location：Cell membrane; Single-pass type III membrane protein. Endomembrane system; Single-pass type III membrane protein.
Tissue Specificity：Expressed in mature B-cells, but not in T-cells or monocytes.
DISEASE：Note=A chromosomal aberration involving TNFRSF17 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with IL2.
Similarity：Contains 1 TNFR-Cys repeat.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
说明书
联系我们
打印此页面
收藏