| Rabbit Anti-PDE5A antibody |
| 反应物种（预测） |
Mouse,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep,GuineaPig |
| 产品应用（已验证） |
WB,IHC,FCM |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=0.2μg/Test,IF=1:100-500, |
| 研究领域 |
肿瘤,心血管,细胞生物,免疫学,染色质和核信号,信号转导,新陈代谢, |
| 标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (rat heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PDE5A) Polyclonal Antibody, Unconjugated (bs-2349R) at 1400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.
Isotype Control Antibody: Rabbit IgG(orange) ;
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue),
Dilution: 1:200 in 1 X PBS containing 0.5% BSA ;
Primary Antibody Dilution: 0.2 μg in 100 μL1X PBS containing 0.5% BSA(green).
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Sample:
Lane 1: HT1080 (Human) Cell Lysate at 30 ug
Lane 2: SH-SY5Y (Human) Cell Lysate at 30 ug
Primary: Anti-PDE5A (bs-2349R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 98 kD
Observed band size: 110 kD
RRID：AB_10885145
产品名称：Rabbit Anti-PDE5A antibody
别名： 5''-cyclic phosphodiesterase; CGB PDE; CGB-PDE; CGBPDE; cGMP binding cGMP specific 3' 5' cyclic nucleotide phosphodiesterase; cGMP binding cGMP specific 35 cyclic nucleotide phosphodiesterase; cGMP binding cGMP specific phosphodiesterase; cGMP binding/cGM
中文名称：磷酸二酯酶5A抗体
英文名称：Rabbit Anti-PDE5A antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human PDE5A
抗原表位：101-200/875
SWISS：O76074
Gene ID ：8654
Human Gene ID：8654
The cGMP specific phosphodiesterase type 5A (PDE5A) family is comprised of 2 genes (PDE5A and PDE5B) each with multiple splice variants generated by RNA splicing and use of alternate initiation sites. The PDE5 is highly expressed in aorta and lungs, intestine, kidney adrenal gland cerebellum and cerebrum. In cerebellum the PDE5 is highly expressed during neonatal development in Purkinge cells layer. The PDE5 is also abundant in vascular smooth muscle regulating cGMP levels and vascular smooth muscle tonicity. In corpus cavernosum inhibition of PDE5 by sildenafil corrects erectile dysfunctions. The nitric oxide donor sodium notropurside (SNP) stimulate PDE5 activity by cGMP dependent kinase phosphorylation. PDE5A1 has 875 amino acids (99.5 kDa). The amino terminal 142 amino acid of the PDE5 gene showed no sequence homology with other PDEs and also contained serine 92 that is phosphorylated by cGMP kinases.
Function：Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP.
Tissue Specificity：Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.
Post-translational modifications：Phosphorylation is regulated by binding of cGMP to the two allosteric sites (By similarity). Phosphorylation by PRKG1 leads to its activation.
Similarity：Belongs to the cyclic nucleotide phosphodiesterase family.
Contains 2 GAF domains.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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