Rabbit Anti-LIPC antibody |
反应物种(预测) |
Dog,Cow,Sheep |
产品应用(已验证) |
WB |
产品应用(可尝试) |
IHC,ICC,IF,ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500, |
研究领域 |
心血管,细胞生物,免疫学,新陈代谢, |
标签 |
Array |
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Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-LIPC Polyclonal Antibody, Unconjugated(bs-18288R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Mouse Kidney tissue lysates
Lane 3: Rat Liver tissue lysates
Lane 4: Rat Kidney tissue lysates
Primary: Anti-LIPC (bs-18288R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
RRID:RRID
产品名称:Rabbit Anti-LIPC antibody
别名: HDLCQ12;
Hepatic lipase;
Hepatic triacylglycerol lipase;
HL;
HTGL;
Lipase member C;
lipC;
LIPC_HUMAN;
LIPH.
中文名称:肝脂酶抗体
英文名称:Rabbit Anti-LIPC antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:分泌型蛋白
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human LIPC
抗原表位:301-400/499
SWISS:P11150
Gene ID :3990
Human Gene ID:3990
LIPC encodes hepatic triglyceride lipase, which is expressed in liver. LIPC has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. [provided by RefSeq, Jul 2008]
Function:Hepatic lipase has the capacity to catalyze hydrolysis of phospholipids, mono-, di-, and triglycerides, and acyl-CoA thioesters. It is an important enzyme in HDL metabolism. Hepatic lipase binds heparin.
Subcellular Location:Secreted.
DISEASE:Defects in LIPC are the cause of hepatic lipase deficiency (HL deficiency) [MIM:151670].
Similarity:Belongs to the AB hydrolase superfamily. Lipase family.
Contains 1 PLAT domain.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.