Rabbit Anti-FHOD1 antibody,蛋白Marker-蛋白检测试剂盒-IVD原料-北京博奥森生物技术有限公司

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Rabbit Anti-FHOD1 antibody

多克隆 | SKU：bs-13158R

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货号:bs-3351R

￥1280

50μg

RMB1280

100μg

RMB1980

200μg

RMB2980

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产品信息
免疫原信息
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订购号:bs-13158R

￥1138.00-2900.00

50ul

100ul

200ul

货期：现货

售后保障

Rabbit Anti-FHOD1 antibody
反应物种（预测）	Dog,Pig,Horse,Rabbit
产品应用（已验证）	WB,FCM
产品应用（可尝试）	IHC,IF,ELISA
推荐稀释比例	WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=2ug/Test,IF=1:100-500,
研究领域	细胞生物,发育生物学,信号转导,细胞骨架,
标签	Array

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-FHOD1 Polyclonal Antibody, Unconjugated(bs-13158R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Sample:
Hela(Human) Cell Lysate at 30 ug
293T(Human) Cell Lysate at 30 ug
Primary: Anti- FHOD1 (bs-13158R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 126 kD
Observed band size: 126 kD
Blank control:MCF7.
Primary Antibody (green line): Rabbit Anti-FHOD1 antibody (bs-13158R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Sample:
Muscle (Mouse) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti- FHOD1 (bs-13158R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 126 kD
Observed band size: 126 kD

产品信息

RRID：RRID
产品名称：Rabbit Anti-FHOD1 antibody
别名： FH1/FH2 domain containing protein; FH1/FH2 domain-containing protein 1; Fhod1; FHOD1_HUMAN; FHOS; FHOS1; Formin homolog overexpressed in spleen 1; Formin homology 2 domain containing 1; Formin homology 2 domain containing protein 1; Formin homology 2 doma
中文名称：肢体畸形相关蛋白FHOD1抗体
英文名称：Rabbit Anti-FHOD1 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆
性状：Liquid
亚型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

免疫原信息

免疫原：KLH conjugated synthetic peptide derived from human FHOD1
抗原表位：601-700/1164
SWISS：Q9Y613
Gene ID ：29109
Human Gene ID：29109

产品介绍

The limb deformity (ld) locus influences normal limb development and gives rise to alternative mRNAs that can translate into a family of protein products known as formins. Formins play a crucial role in cytoskeletal reorganization by influencing actin filament assembly. The temporal genetic hierarchy influencing normal limb development can deregulate and mediate mammalian developmental syndromes. FHOD1 induces the formation of and associates with bundled actin stress fibers in response to the activity of the Rho-ROCK cascade. It influences several cellular activities including cell migration, cytoskeletal arrangement, signal transduction and gene expression.
Function：Required for the assembly of F-actin structures, such as stress fibers. Depends on the Rho-ROCK cascade for its activity. Contributes to the coordination of microtubules with actin fibers and plays a role in cell elongation.
Subunit：Self-associates via the FH2 domain. Binds to F-actin via its N-terminus. Binds to the cytoplasmic domain of CD21 via its C-terminus. Interacts with ROCK1 in a Src-dependent manner.
Subcellular Location：Cytoplasm. Cytoplasm; cytoskeleton. Predominantly cytoplasmic.
Tissue Specificity：Ubiquitous. Highly expressed in spleen.
Post-translational modifications：Phosphorylated by ROCK1.
Similarity：Belongs to the formin homology family.
Contains 1 FH1 (formin homology 1) domain.
Contains 1 FH2 (formin homology 2) domain.
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.