| Rabbit Anti-KLK11 antibody |
| 反应物种（预测） |
Mouse,Rat,Dog |
| 产品应用（已验证） |
FCM |
| 产品应用（可尝试） |
WB,IHC,IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=2ug/Test,IF=1:100-500, |
| 研究领域 |
细胞生物,免疫学,激酶和磷酸酶 |
| 标签 |
Array |
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Blank control:A431.
Primary Antibody (green line): Rabbit Anti-KLK11 antibody (bs-3799R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID：AB_10857698
产品名称：Rabbit Anti-KLK11 antibody
别名： Hippostasin; hK11; KLK 11; KLK-11; kallikrein 11; Kallikrein 11 isoform 1 preproprotein; Kallikrein 11 isoform 2 precursor; Kallikrein 11 precursor; kallikrein related peptidase 11; KLK 11; KLK11; Protease serine 20 trypsin like; Protease serine trypsin l
中文名称：激肽释放酶11抗体
英文名称：Rabbit Anti-KLK11 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆,分泌型蛋白
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human KLK11
抗原表位：201-282/282
SWISS：Q9UBX7
Gene ID ：11012
Human Gene ID：11012
Kallikrein 11 has two alternative splicing isoforms, known as the brain type and prostate type. Elevated serum levels of hK11 have been found in 70% of women with ovarian cancer and in 60% of men with prostate cancer. Analysis of this biomarker in serum may aid in the diagnosis and monitoring of ovarian and prostatic carcinoma.
Function：Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues.
Subcellular Location：Isoform 1: Secreted.
Isoform 2: Golgi apparatus.
Tissue Specificity：Expressed in brain, skin and prostate. Isoform 1 is expressed preferentially in brain. Isoform 2 is expressed in prostate. Present in seminal plasma at concentrations ranging from 2 to 37 microg/mL (at protein level).
Post-translational modifications：About 40% of KLK11 is inactivated by internal cleavage after Arg-188. This proteolytic inactivation may be effected by plasminogen.
Similarity：Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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