| Rabbit Anti-GLUT12 antibody |
| 反应物种（预测） |
Chicken,Dog,Pig,Cow,Horse,Rabbit,GuineaPig |
| 产品应用（已验证） |
WB,IHC,ICC |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100, |
| 研究领域 |
免疫学,转运蛋白, |
| 标签 |
Array |
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Paraformaldehyde-fixed, paraffin embedded (mouse spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLUT12) Polyclonal Antibody, Unconjugated (bs-2540R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLUT12) Polyclonal Antibody, Unconjugated (bs-2540R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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SHSY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GLUT12) polyclonal Antibody, Unconjugated (bs-2540R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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Sample:
Heart (Rat) Lysate at 40 ug
Primary: Anti- GLUT12 (bs-2540R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68kD
Observed band size: 68kD
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Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLUT12) Polyclonal Antibody, Unconjugated (bs-2540R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Tissue/cell: mouse heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Glut12 Polyclonal Antibody, Unconjugated(bs-2540R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
RRID：AB_10855200
产品名称：Rabbit Anti-GLUT12 antibody
别名： GLUT-12; GTR12_HUMAN; Glucose transporter type 12; Glucose transporter type 12; GLUT 12; GLUT8; SLC2A12; Solute carrier family 2 (facilitated glucose transporter) member 12; Solute carrier family 2 facilitated glucose transporter member 12; Solute carrier
中文名称：葡萄糖转运蛋白12抗体
英文名称：Rabbit Anti-GLUT12 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆,细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human GLUT12
抗原表位：251-350/617
抗原细胞定位：Extracellular
SWISS：Q8TD20
Gene ID ：154091
Human Gene ID：154091
GLUT12 belongs to a family of transporters that catalyze the uptake of sugars through facilitated diffusion. Thirteen different types of glucose/fructose transport carrier proteins designated as Glut 1-13 facilitate glucose/fructose transport across the cell membrane. Individual members of the Glut family have predicted secondary structure characteristic of 12 membrane spanning domains of other transport carriers.
Function：Facilitative glucose transporter.
Subcellular Location：Endomembrane system; Multi-pass membrane protein. Cytoplasm, perinuclear region. Note=Localizes primarily perinuclear region in the absence of insulin.
Tissue Specificity：Predominantly expressed in skeletal muscle, heart and prostate, with lower levels in brain, placenta and kidney.
Similarity：Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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