| Rabbit Anti-PKA C alpha + beta antibody |
| 反应物种（预测） |
Chicken,Dog,Pig,Cow |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500, |
| 研究领域 |
肿瘤,免疫学,神经生物学,信号转导,转录调节因子,激酶和磷酸酶 |
| 标签 |
Array |
-
Sample:
Brain(Rat) Lysate at 40 ug
Primary: Anti-PKA C alpha+beta (bs-2371R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40/50 kD
Observed band size: 50 kD
-
Sample:
Testis (Mouse) Lysate at 40 ug
Testis(Rat) Lysate at 40 ug
Primary: Anti-PKA C alpha+beta (bs-2371R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40/50 kD
Observed band size: 50 kD
-
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PKA C alpha+beta) Polyclonal Antibody, Unconjugated (bs-2371R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID：AB_10855968
产品名称：Rabbit Anti-PKA C alpha + beta antibody
别名： PKA C alpha+beta; PKA C alpha/beta; PKA C beta; PKACB; KAPCB_HUMAN; PRKACB; Protein kinase cAMP dependent catalytic beta; KAPCA_HUMAN; cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha.
中文名称：蛋白激酶C抗体
英文名称：Rabbit Anti-PKA C alpha + beta antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆,细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human PKA beta
抗原表位：251-351/351
SWISS：P17612
Gene ID ：5566
Human Gene ID：5566
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP dependent protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. PKA beta (catalytic subunit) is a member of the Ser/Thr protein kinase family and is a catalytic subunit of AMPK.
Function：Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and de
Subunit：A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regul
Subcellular Location：oplasm. Cell membrane. Nucleus (By similarity). Note=Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm (By similarity).
Tissue Specificity：Isoform 1 is most abundant in the brain, with low level expression in kidney. Isoform 2 is predominantly expressed in thymus, spleen and kidney. Isoform 3 and isoform 4 are only expressed in the brain.
Post-translational modifications：Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively (By similarity).
Similarity：Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
说明书
联系我们
打印此页面
收藏