| Rabbit Anti-MYLPF antibody |
| 反应物种（预测） |
Dog,Pig,Cow,Horse,Rabbit,Sheep |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
IF,ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500, |
| 研究领域 |
免疫学,信号转导,细胞骨架,细胞外基质, |
| 标签 |
Array |
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Tissue/cell: muscle of mouse embryo; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Fast skeletal Myosin Polyclonal Antibody, Unconjugated(bs-5159R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MYLPF) Polyclonal Antibody, Unconjugated (bs-5159R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Sample:
A673(Human) Cell Lysate at 30 ug
Primary: Anti-MYLPF (bs-5159R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19 kD
Observed band size: 19 kD
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Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-MYLPF (bs-5159R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19 kD
Observed band size: 19 kD
RRID：AB_11048731
产品名称：Rabbit Anti-MYLPF antibody
别名： 2410014J02Rik;
DKFZp779C0757;
DTNB;
Fast skeletal myosin light chain 2;
G2;
HUMMLC2B;
MGC13450;
MLC 2; MRLC2; MYL11;
MLC2B;
MLC2F;
MLRS_HUMAN;
MYLPF;
Myosin light polypeptide 2 alkali;
Myosin regulatory light chain 2, skeletal muscle isoform.
中文名称：快速骨骼肌肌球蛋白轻链2抗体
英文名称：Rabbit Anti-MYLPF antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞浆
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human Fast skeletal myosin light chain 2
抗原表位：101-170/170
SWISS：Q96A32
Gene ID ：29895
Human Gene ID：29895
Myosin is a highly conserved, ubiquitously expressed protein that interacts with Actin to generate the force for cellular movements. Conventional Myosins are hexameric proteins consisting of two heavy chain subunits, a pair of non-phosphorylatable light chain subunits and a pair of phosphorylatable light chain subunits. Three general classes of Myosin have been cloned: smooth muscle Myosins, striated muscle Myosins and non-muscle Myosins . Contractile activity in smooth muscle is regulated by the calcium/calmodulin-dependent phosphorylation of Myosin light chain (MLC) by Myosin light chain kinase. Myosin heavy chains, which are encoded by the MYH gene family, contain Actin-activated ATPase activity which generates the motor function of Myosin. Myosin heavy chains were initially isolated from a human fetal skeletal muscle and are the major determinant in the speed of contraction of skeletal muscle. Various isoforms of myosin heavy chains are differentially expressed depending on the functional activity of the muscle.
Subunit：Myosin is a hexamer of 2 heavy chains and 4 light chains.
Tissue Specificity：Expressed in fetal and adult skeletal muscle.
Similarity：Contains 3 EF-hand domains.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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