Rabbit Anti-Endothelin B Receptor antibody |
反应物种(预测) |
Chicken,Dog,Pig,Cow,Horse,Rabbit |
产品应用(已验证) |
WB |
产品应用(可尝试) |
ELISA |
推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000, |
研究领域 |
心血管,免疫学,信号转导,G蛋白偶联受体, |
标签 |
Array |
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Overlay histogram showing SP2/0 cells stained with bs-4198R-PE (red line). The cells were fixed with 1%paraformaldehyde (10 min) and then permeabilized with 1x PBS /0.2%TritonX-100 for 5 min. The cells were then incubated in 1x PBS / 1%BSA to block non-specific protein-protein interactions. The cells were then incubated with the antibody (bs-4198R-PE, 2ug/1x106 cells) for 30 min at 22-25°C. Isotype control antibody (black line) was rabbit IgG (2ug/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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Sample:
Lane1:Heart (Rat)Lysate at 40 ug
Lane2:Heart (Mouse) Lysate at 40 ug
Primary: Anti-Endothelin B Receptor(bs-4198R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 47kD
Observed band size: 50kD
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Sample:
Lane 1: Embryo (Mouse) Tissue Lysate at 40 ug
Lane 2: Lung (Mouse) TissueLysate at 40 ug
Lane 3: Cerebrum (Mouse) Tissue Lysate at 40 ug
Lane 4: Cerebrum (Rat) Tissue Lysate at 40 ug
Lane 5: A431 (Human) Cell Lysate at 30 ug
Lane 6: BV-2 (Mouse) Cell Lysate at 30 ug
Lane 7: SH-SY5Y (Human) Cell Lysate at 30 ug
Primary: Anti-Endothelin B Receptor (bs-4198R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 50 kD
RRID:AB_11076171
产品名称:Rabbit Anti-Endothelin B Receptor antibody
别名: ABCDS; Ednra; Ednrb; EDNRB_HUMAN; Endothelin B receptor; Endothelin B receptor precursor; Endothelin receptor Non selective type; Endothelin receptor non-selective type; Endothelin receptor type B; ET B; ET-B; ET-BR; ETB; ETRB; Hirschsprung disease 2; HSC
中文名称:内皮素B受体抗体
英文名称:Rabbit Anti-Endothelin B Receptor antibody
抗体来源: Rabbit
克隆类型:多克隆
细胞定位:细胞膜
性 状:Liquid
亚 型:IgG
纯化方法:affinity purified by Protein A
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原:KLH conjugated synthetic peptide derived from human Endothelin B Receptor
抗原表位:378-442/442
抗原细胞定位:Cytoplasmic
SWISS:P24530
Gene ID :1910
Human Gene ID:1910
The protein encoded by this gene is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutations in the endothelin receptor type B gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Function:Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Subcellular Location:Cell membrane; Multi-pass membrane protein.
Tissue Specificity:Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells.
Post-translational modifications:Palmitoylation of Cys-402 was confirmed by the palmitoylation of Cys-402 in a deletion mutant lacking both Cys-403 and Cys-405.
DISEASE:Defects in EDNRB are a cause of Waardenburg syndrome type 4A (WS4A) [MIM:277580]; also known as Waardenburg-Shah syndrome. WS4A is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in
Similarity:Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRB sub-subfamily.
Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.