| Rabbit Anti-CD44v4 antibody |
| 反应物种（预测） |
Dog,Horse,Rabbit |
| 产品应用（已验证） |
ICC,FCM |
| 产品应用（可尝试） |
WB,IHC,IF,ELISA |
| 推荐稀释比例 |
WB=,Elisa=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg /Test,IF=1:100-500,ICC=1:100, |
| 研究领域 |
肿瘤,细胞生物,细胞膜受体,细胞表面分子,糖蛋白,内皮细胞, |
| 标签 |
Array |
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Blank control(blue): U251 (fixed with Ice-cold 70% ethanol overnight at 4 °C).
Primary Antibody:Rabbit Anti-CD44v4 antibody (bs-2783R,Green); Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (CD44v4) polyclonal Antibody, Unconjugated (bs-2783R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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Tissue/cell:A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (CD44v4) polyclonal Antibody, Unconjugated (bs-2783R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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Blank control: HELA.
Primary Antibody (green line): Rabbit Anti-CD44v4 antibody (bs-2783R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
RRID：AB_10854140
产品名称：Rabbit Anti-CD44v4 antibody
别名： CD44 antigen; CD44 antigen isoform 4 precursor; CD44 molecule; Heparan sulfate proteoglycan; PGP1; CDW44; CSPG8; ECMR-III; HCELL; HUTCH-I; IN; LHR; MC56; MDU2; MDU3; MGC10468; MIC4; Pgp1; CD44 antigen isoform 4 precursor; hematopoietic cell E- and L-selec
中文名称：CD44V4抗体
英文名称：Rabbit Anti-CD44v4 antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞膜
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human CD44v4
抗原表位：141-240/361
SWISS：P16070
Gene ID ：960
Human Gene ID：960
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq].
Function：Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA
Subcellular Location：Membrane.
Tissue Specificity：Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
Post-translational modifications：Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the a
Similarity：Contains 1 Link domain.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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