| Rabbit Anti-Calbindin antibody |
| 反应物种（预测） |
Dog,Pig,Cow,Horse,Rabbit |
| 产品应用（已验证） |
WB,IHC |
| 产品应用（可尝试） |
ELISA |
| 推荐稀释比例 |
WB=1:500-2000,Elisa=1:5000-10000,IHC-P=1:100-500, |
| 研究领域 |
细胞生物,免疫学,神经生物学,生长因子和激素,结合蛋白, |
| 标签 |
Array |
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Sample:
kidney (mouse) Lysate at 40 ug
brain (Mouse) Lysate at 40 ug
Primary: Anti-Calbindin(bs-10177R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size: 29 kD
Observed band size: 29 kD
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Sample:
Lane 1: Mouse Brain Lysates
Lane 2: Mouse Cerebellum Lysates
Lane 3: Mouse Kidney Lysates
Lane 4: Rat Kidney Lysates
Primary: Anti-Calbindin (bs-10177R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29kDa
Observed band size: 29kDa
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Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calbindin) Polyclonal Antibody, Unconjugated (bs-10177R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calbindin) Polyclonal Antibody, Unconjugated (bs-10177R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calbindin) Polyclonal Antibody, Unconjugated (bs-10177R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calbindin) Polyclonal Antibody, Unconjugated (bs-10177R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calbindin) Polyclonal Antibody, Unconjugated (bs-10177R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Calbindin) Polyclonal Antibody, Unconjugated (bs-10177R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
RRID：RRID
产品名称：Rabbit Anti-Calbindin antibody
别名： CAB27; CALB 1; CALB; CALB1; Calbindin 1 28kDa; Calbindin-D-28K; Calbindin D28; D 28K; D28K; Vitamin D dependent calcium binding protein; Vitamin D dependent calcium binding protein avian type; Vitamin D dependent calcium binding protein avian-type; Vitami
中文名称：钙结合蛋白D28K抗体
英文名称：Rabbit Anti-Calbindin antibody
抗体来源： Rabbit
克隆类型：多克隆
细胞定位：细胞核,细胞浆,细胞外基质
性 状：Liquid
亚 型：IgG
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
免 疫 原：KLH conjugated synthetic peptide derived from human Calbindin
抗原表位：41-150/261
SWISS：P05937
Gene ID ：793
Human Gene ID：793
Calbindin is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27-kD protein induced by vitamin D in the duodenum of the chick. In the brain, its synthesis is independent of vitamin-D-derived hormones. Calbindin contains 4 active calcium-binding domains, and 2 modified domains that presumably have lost their calcium-binding capacity. The neurons in brains of patients with Huntington disease are calbindin-depleted. [provided by RefSeq, Jul 2008]
Function：Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
Subunit：Interacts with RANBP9.
Similarity：Belongs to the calbindin family.
Contains 5 EF-hand domains.
Important Note：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
400-901-9800
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